科研项目

1.      国家自然科学基金-面上项目，表面增强拉曼光谱及成像技术在肿瘤细胞检测中的应用研究，2016-01至2019-12，主持

2.      国家自然科学基金-青年基金，基于表面增强拉曼光谱检测生物标志物技术的研究，2013-01至2015-12，主持

3.      山东省高等学校科技计划项目，肿瘤生物标志物拉曼光谱传感方法的研究与应用，2017-01至2018-12，主持

4.      国家自然科学基金-重点项目，靶向肺癌循环肿瘤细胞的单分子检测技术研究，2018-01至2022-12，参加

5.      江苏省前沿技术研发计划项目，靶向乳腺癌表观遗传修饰的单分子检测技术研发，2024-09至2027-09，参加

论文

1.      Dongling Li, Jinping Hu, Juan Hu,* Chun-yang Zhang,* Integration of AND logic circuit with CRISPR/Cas12a system for sensitive detection of biomarkers and accurate discrimination of breast cancer cells. Biosensors and Bioelectronics, 2025, 288, 117790. (JCR一区, IF: 10.5)

2.      Jinping Hu, Jie Zhang, Juan Hu,* Chun-yang Zhang,* Construction of a dual-color fluorescent light-up biosensor based on sequential coding for label-free and sensitive detection of multiple Piwi-interacting RNAs. Chemical Communications, 2025, 61(26), 5031-5034. (JCR二区, IF: 4.2)

3.      Huimin Yuan, Qi-qin Ge, Jinping Hu, Xiao-fei Liu, Juan Hu,* Chun-yang Zhang,* Integration of biodegradable nanosphere-mediated spatial-localized cascade catalytic hairpin assembly with quantum dots for intracellular circular RNA imaging and clinical diagnosis. Chemical Engineering Journal, 2025, 507, 160685. (JCR一区, IF: 13.2)

4.      Shi Zheng, Jinping Hu, Zichen Jiao, Tao Wang,* Juan Hu,* Chun-yang Zhang,* Lighting up three-dimensional nanolantern for circular RNA imaging and precise gene therapy. Biosensors and Bioelectronics, 2025, 276, 117273. (JCR一区, IF: 10.5)

5.      Ze Wang, Wen-jing Liu, Jinqiu Tao, Juan Hu,* Chun-yang Zhang,* Enzymatic cascade amplification-modulated Thermus thermophilus Argonaute biosensor for simultaneous monitoring of multiple Piwi-interacting RNAs. Biosensors and Bioelectronics, 2025, 276, 117261. (JCR一区, IF: 10.5)

6.      Juan Hu, Yue-ying Li, Su Jiang, Wei Gao,* Chun-yang Zhang,* Ligation-driven hyperbranched DNA assembly for label-free and sequence-specific measurement of RNA N6-methyladenosine in human breast tissues, Sensors and Actuators B: Chemical, 2025, 422, 136635. (JCR一区, IF: 7.7)

7.      Jie Zhang, Jiawen Liu, Lixue Qiao, Qian Zhang,* Juan Hu,* Chun-yang Zhang, Recent Advance in Single-Molecule Fluorescent Biosensors for Tumor Biomarker Detection, Biosensors, 2024, 14(11), 540. (JCR一区, IF: 5.6)

8.      Wen-jing Liu, Hai-juan Li, Jinqiu Tao, Lu-yao Wang, Juan Hu,* Chun-yang Zhang,* High-fidelity CRISPR/Cas13a trans cleavage-driven assembly of single quantum dot nanosensor for ultrasensitive detection of long noncoding RNAs in clinical breast tissues, Nano Today, 2024, 59, 102529. (JCR一区, IF: 10.9)

9.      Huimin Yuan, Wen-jing Liu, Juan Hu,* Chun-yang Zhang,* Recent advances in single-nucleotide variant assay: From in vitro detection to in vivo imaging, TrAC Trends in Analytical Chemistry, 2024, 180, 117963. (JCR一区, IF: 12)

10.   Yun Han, Su Jiang, Peng-yu Wang, Juan Hu,* Chun-yang Zhang,* Autonomous enzymatic synthesis of functional nucleic acids for sensitive measurement of long noncoding RNA in human lung tissues, Talanta, 2024, 274, 126030. (JCR一区, IF: 6.1)

11.   Qian Zhang, Juan Hu, Dong-ling Li, Jian-Ge Qiu, Bing-Hua Jiang,* Chun-yang Zhang,* Construction of single-molecule counting-based biosensors for DNA-modifying enzymes: A review, Analytica Chimica Acta, 2024, 342395. (JCR一区, IF: 6)

12.   Maoding Zuo, Yuncong Yang, Su Jiang, Chenyu Zhu, Yun Han, Juan Hu, Kewei Ren,* Lin Cui,* Chun-Yang Zhang,* Ultrathin-FeOOH-coated MnO2 nanozyme with enhanced catalase-like and oxidase-like activities for photoelectrochemical and colorimetric detection of organophosphorus pesticides, Food Chemistry, 2024, 445, 138716. (JCR一区, IF: 9.8)

13.   Meng Liu, Zi-wei Han, Su Jiang, Yun Han, Hao Liu, Di Zhang, Juan Hu,* Qinfeng Xu,* Chun-yang Zhang,* Construction of a transcription-driven CRISPR RNA auto-generation-mediated CRISPR-Cas12a system for sensitive detection of endogenous repair glycosylase, Sensors and Actuators B: Chemical, 2024, 405, 135401. (JCR一区, IF: 7.7)

14.   Jin-zhi Zhang, Ning-ning Zhao, Zi-yue Wang, Juan Hu,* Chun-yang Zhang,* Target-activated T7 transcription circuit-mediated multiple cycling signal amplification for monitoring of flap endonuclease 1 activity in cancer cells. Analyst, 2023, 148(12), 2732-2738. (JCR二区, IF: 3.3)

15.   Shuangshuang Zhang, Jin-an Hou, Juan Hu,* Chun-Yang Zhang,* In-situ self-catalyzed growth of manganese embedded 3D flakes-coated carbon rod as an efficient oxygen reduction reaction catalyst of zinc-air batteries. Chemistry-A European Journal, 2023, 29(5), e202202989. (JCR二区, IF: 3.7)

16.   Xiao-wen Liu, Wen-jing Liu, Yingying Meng, Juan Hu,* Chun-yang Zhang,* Development of a tandem signal amplification strategy for label-free sensing polynucleotide kinase activity in cancer cells. Talanta, 2023, 253, 124001. (JCR一区, IF:6.1)

17.   Lin Cui, Chen-yu Zhu, Juan Hu, Xiao-meng Meng, Mengmeng Jiang, Wenqiang Gao,* Xiaolei Wang,* Chun-yang Zhang,* Construction of a dual-mode biosensor for electrochemiluminescent and electrochemical sensing of alkaline phosphatase. Sensors and Actuators B-Chemical, 2023, 374, 132779. (JCR一区, IF: 7.7)

18.   Su Jiang, Qian Liu, Juan Hu, Dong Yuan, Yan Zhang,* Chun-yang Zhang,* Target-triggered assembly of functional G-quadruplex DNAzyme nanowires for sensitive detection of miRNA in lung tissues. Sensors and Actuators B-Chemical, 2022, 373, 132689. (JCR一区, IF: 7.7)

19.   Zi-yue Wang, Huimin Yuan, Dong-ling Li, Juan Hu,* Jian-Ge Qiu,* Chun-yang Zhang,* Hydroxymethylation-specific ligation-mediated single quantum dot-based nanosensors for sensitive detection of 5-hydroxymethylcytosine in cancer cells. Analytical Chemistry, 2022, 94(27), 9785-9792. (JCR一区, IF: 6.7)

20.   Wen-jing Liu, Xinyi Zhang, Juan Hu,* Chun-yang Zhang,* A label-free and self-circulated fluorescent biosensor for sensitive detection of ten-eleven translocation 1 in cancer cells. Chemical Communications, 2022, 58(57), 7996-7999. (JCR二区, IF: 4.2)

21.   Shu-hua Wei, Meng Liu, Juan Hu,* Chun-yang Zhang,* Target-initiated cascade signal amplification lights up a G-quadruplex for a label-free detection of circular ribonucleic acids. Analytical Chemistry, 2022, 94(25), 9193-9200. (JCR一区, IF: 6.7)

22.   Xue-ru Wei, Yingying Meng, Qinfeng Xu, Juan Hu,* Chun-yang Zhang,* Label-free and homogeneous detection of flap endonuclease 1 by ligation-promoted hyperbranched rolling circle amplification platform. Talanta, 2022, 243, 123342. (JCR一区, IF: 6.1)

23.   Yan Zhang, Chen Wang, Xiaoran Zou, Xiaorui Tian, Juan Hu,* Chun-yang Zhang,* Simultaneous enzyme-free detection of multiple long noncoding RNAs in cancer cells at single-molecule/particle level. Nano Letters, 2021, 21(10), 4193-4201. (JCR一区, IF: 9.1)

24.   Chen-chen Li, Hui-yan Chen, Xiliang Luo,* Juan Hu,* Chun-yang Zhang,* Multicolor fluorescence encoding of different microRNAs in lung cancer tissues at the single-molecule level. Chemical Science, 2021, 12(37), 12407-12418. (JCR一区, IF: 7.4)

25.   Chen-chen Li, Jinping Hu, Xiliang Luo,* Juan Hu,* Chun-yang Zhang,* Development of a single quantum dot-mediated FRET nanosensor for sensitive detection of single-nucleotide polymorphism in cancer cells. Analytical Chemistry, 2021, 93(43), 14568-14576. (JCR一区, IF: 6.7)

26.   Chen-chen Li, Yue-hong Dong, Xiaoran Zou, Xiliang Luo,* Dazhong Shen,* Juan Hu,* Chun-yang Zhang,* Label-free and template-free chemiluminescent biosensor for sensitive detection of 5-hydroxymethylcytosine in genomic DNA. Analytical Chemistry, 2021, 93(4), 1939-1943. (JCR一区, IF: 6.7)

27.   Juan Hu, Wen Liu, Jufeng Wang, Jian-Ge Qiu,* Chun-yang Zhang,* Simple mix-and-read assay with multiple cyclic enzymatic repairing amplification for rapid and sensitive detection of DNA glycosylase. Analytical Chemistry, 2021, 93(18), 6913-6918. (JCR一区, IF: 6.7)

28.   Juan Hu, Jie Yao, Jufeng Wang, Li-yuan Pan, Jian-Ge Qiu,* Chun-yang Zhang,* A single quantum dot-based fluorescence resonance energy transfer biosensor for antibody-free detection of ten-eleven translocation 1. Chemical Communications, 2021, 57(29), 3543-3546. (JCR二区, IF: 4.2)

29.   Juan Hu, Li-yuan Pan, Yueying Li, Xiaoran Zou, Bing-jie Liu, BingHua Jiang,* Chun-yang Zhang,* Deacetylation-activated construction of single quantum dot-based nanosensor for sirtuin 1 assay. Talanta, 2021, 224, 121918. (JCR一区, IF: 6.1)

30.   Junjie Zhang, Xianwei Su, Ao Qi, Lulu Liu, Lijian Zhang, Yu Zhong, Simin Xu, Ximei Liu, Juan Hu,* Yusen Chen,* Chun-yang Zhang,* Chun Cai,* Metabolomic profiling of fatty acid biomarkers for intracerebral hemorrhage stroke. Talanta, 2021, 222, 121679. (JCR一区, IF: 6.1)

31.   Chen-chen Li, Hui-yan Chen, Yue-hong Dong, Xiliang Luo,* Juan Hu,* Chun-yang Zhang,* Advances in detection of epigenetic modification-5-hydroxymethylcytosine. Acta Chimica Sinica, 2021, 79(5), 614-627. (JCR三区, IF: 1.6)

32.   Chen-chen Li, Hui-yan Chen, Juan Hu,* Chun-yang Zhang,* Rolling circle amplification-driven encoding of different fluorescent molecules for simultaneous detection of multiple DNA repair enzymes at the single-molecule level. Chemical Science, 2020, 11(22), 5724-5734. (JCR一区, IF: 7.4)

33.   Yueying Li, Wen Liu, Qinfeng Xu, Juan Hu,* Chun-yang Zhang,* Construction of a sensitive protease sensor with DNA-peptide conjugates for single-molecule detection of multiple matrix metalloproteinases. Biosensors and Bioelectronics, 2020, 169, 112647. (JCR一区, IF: 10.5)

34.   Juan Hu, Yang Liu, Chun-yang Zhang,* Construction of a single quantum dot nanosensor with the capability of sensing methylcytosine sites for sensitive quantification of methyltransferase. Nanoscale, 2020, 12(7), 4519-4526. (JCR一区, IF: 5.1)

35.   Juan Hu, Wen-can Li, Jian-Ge Qiu, BingHua Jiang,* Chun-yang Zhang,* A multifunctional DNA nanostructure based on multicolor FRET for nuclease activity assay. Analyst, 2020, 145(18), 6054-6060. (JCR二区, IF: 3.3)

36.   Juan Hu, Ming-hao Liu, Chun-yang Zhang,* Construction of tetrahedral DNA-quantum dot nanostructure with the integration of multistep Förster resonance energy transfer for multiplex enzymes assay. ACS Nano, 2019, 13(6), 7191-7201. (JCR一区, IF: 16)

37.   Chen-chen Li, Wan-xin Liu, Juan Hu,* Chun-yang Zhang,* A single quantum dot-based nanosensor with multilayer of multiple acceptors for ultrasensitive detection of human alkyladenine DNA glycosylase. Chemical Science, 2019, 10(37), 8675-8684. (JCR一区, IF: 7.4)

38.   Lin Cui, Juan Hu, Meng Wang, Chen-chen Li, Chun-yang Zhang,* Label-free and immobilization-free electrochemical magnetobiosensor for sensitive detection of 5-hydroxymethylcytosine in genomic DNA. Analytical Chemistry, 2019, 91(2), 1232-1236. (JCR一区, IF: 6.7)

39.   Juan Hu, Ming-hao Liu, Chun-yang Zhang,* Integration of isothermal amplification with quantum dot-based fluorescence resonance energy transfer for simultaneous detection of multiple microRNAs. Chemical Science, 2018, 9(18), 4258-4267. (JCR一区, IF: 7.4)

40.   Juan Hu, Ming-hao Liu, Ying Li, Bo Tang,* Chun-yang Zhang,* Simultaneous sensitive detection of multiple DNA glycosylases from lung cancer cells at the single-molecule level. Chemical Science, 2018, 9(3), 712-720. (JCR一区, IF: 7.4)

41.   Chen-chen Li, Juan Hu, Mengfei Lu, Chun-yang Zhang,* Quantum dot-based electrochemical biosensor for stripping voltammetric detection of telomerase at the single-cell level. Biosensors and Bioelectronics, 2018, 122, 51-57. (JCR一区, IF: 10.5)

42.   Lin Cui, Juan Hu, Chen-chen Li, Cheng-ming Wang, Chun-yang Zhang,* An electrochemical biosensor based on the enhanced quasi-reversible redox signal of prussian blue generated by self-sacrificial label of iron metal-organic framework. Biosensors and Bioelectronics, 2018, 122, 168-174. (JCR一区, IF: 10.5)

43.   Lin Cui, Juan Hu, Meng Wang, Xing-kang Diao, Chen-chen Li, Chun-yang Zhang,* Mimic peroxidase- and Bi₂S₃ nanorod-based photoelectrochemical biosensor for signal-on detection of polynucleotide kinase. Analytical Chemistry, 2018, 90(19), 11478-11485. (JCR一区, IF: 6.7)

44.   Dandan Zhang, Juan Hu, Xiao-yun Yang, Yanxia Wu, Wenmei Su, Chun-yang Zhang,* Target-initiated synthesis of fluorescent copper nanoparticles for the sensitive and label-free detection of bleomycin. Nanoscale, 2018, 10(23), 11134-11142. (JCR一区, IF: 5.1)

45.   Juan Hu, Yueying Li, Ying Li, Bo Tang,* Chun-yang Zhang,* Single quantum dot-based nanosensor for sensitive detection of O-GIcNAc transferase activity. Analytical Chemistry, 2017, 89(23), 12992-12999. (JCR一区, IF: 6.7)

46.   Juan Hu, Zi-yue Wang, Chen-chen Li, Chun-yang Zhang,* Advances in single quantum dot-based nanosensors. Chemical Communications, 2017, 53(100), 13284-13295. (JCR二区, IF: 4.2)

47.   Juan Hu, Zi-Yue Wang, Chun-Yang Zhang,* Advance in quantitative single-molecule detection. Scientia Sinica Chimica, 2017, 47(10), 1161-1169.

48.   Si-qiang Huang, Juan Hu, Guichi Zhu, Chun-yang Zhang,* Sensitive detection of point mutation using exponential strand displacement amplification-based surface enhanced Raman spectroscopy. Biosensors and Bioelectronics, 2015, 65, 191-197. (JCR一区, IF: 10.5)

49.   Li-Ping Ye, Juan Hu, Li Liang, Chun-yang Zhang,* Surface-enhanced Raman spectroscopy for simultaneous sensitive detection of multiple microRNAs in lung cancer cells. Chemical Communications, 2014, 50(80), 11883-11886. (JCR二区, IF: 4.2)

50.   Juan Hu, Chun-yang Zhang,* Multiplex detection of lung cancer cells at the single-molecule level. Chemical Communications, 2014, 50(88), 13581-13584. (JCR二区, IF: 4.2)

51.   Juan Hu, Chun-yang Zhang,* Simple and accurate quantification of quantum yield at the single-molecule/particle level. Analytical Chemistry, 2013, 85(4), 2000-2004. (JCR一区, IF: 6.7)

52.   Ya-ping Zeng, Juan Hu, Yi Long, Chun-yang Zhang,* Sensitive detection of DNA methyltransferase using hairpin probe-based primer generation rolling circle amplification-induced chemiluminescence. Analytical Chemistry, 2013, 85(12), 6143-6150. (JCR一区, IF: 6.7)

53.   Yan Zhang, Juan Hu, Chun-yang Zhang,* Sensitive detection of transcription factors by isothermal exponential amplification-based colorimetric assay. Analytical Chemistry, 2012, 84(21), 9544-9549. (JCR一区, IF: 6.7)

54.   Juan Hu, Chun-yang Zhang,* Single base extension reaction-based surface enhanced Raman spectroscopy for DNA methylation assay, Biosensors and Bioelectronics, 2012, 31(1), 451-457. (JCR一区, IF: 10.5)

55.   Cai-jun Cao, De-rong Li, Chao-xiong Chen, Xiao-yun Yang, Juan Hu, Yong Yang, Chun-yang Zhang,* Multimodal optical microscopy in combination with gold nanorods for cancer cell imaging. Journal of Biomedical Optics, 2012, 17(12), 126002. (JCR二区, IF: 2.9)

56.   Chun-yang Zhang,* Juan Hu, Single quantum dot-based nanosensor for multiple DNA detection. Analytical Chemistry, 2010, 82(5), 1921-1927. (JCR一区, IF: 6.7)

57.   Juan Hu, Chun-yang Zhang,* Sensitive detection of nucleic acids with rolling circle amplification and surface-enhanced Raman scattering spectroscopy, Analytical Chemistry, 2010, 82(21), 8991-8997. (JCR一区, IF: 6.7)

58.   Juan Hu, Chun-yang Zhang,* Surface-enhanced Raman scattering technology and its application to gene analysis. Progress in Chemistry, 2010, 22(8), 1641-1647. (JCR三区, IF: 1.2)

59.   Juan Hu, Peng-Cheng Zheng, Jian-Hui Jiang, Guo-Li Shen, Ru-Qin Yu,* Guo-Kun Liu,* Sub-attomolar HIV-1 DNA detection using surface-enhanced Raman spectroscopy. Analyst, 2010, 135(5), 1084-1089. (JCR二区, IF: 3.3)

60.   Juan Hu, Peng-Cheng Zheng, Jian-Hui Jiang, Guo-Li Shen, Ru-Qin Yu,* Guo-Kun Liu,* Electrostatic interaction based approach to thrombin detection by surface-enhanced Raman spectroscopy. Analytical Chemistry, 2009, 81(1), 87-93. (JCR一区, IF: 6.7)

61.   Pengcheng Zheng, Juan Hu, Guoli Shen, Jianhui Jiang, Ruqin Yu,* Guokun Liu,* Synthesis, structure and growth mechanism of size and shape tunable Au/Ag bimetallic nanoparticles. Chinese Journal of Chemistry, 2009, 27(11), 2137-2144. (JCR二区, IF: 5.5)

62.   Guo-Kun Liu, Juan Hu, Peng-Cheng Zheng, Guo-Li Shen, Jian-Hui Jiang, Ru-Qin Yu,* Yan Cui, Bin Ren,* Laser-induced formation of metal-molecule-metal junctions between Au nanoparticles as probed by surface-enhanced Raman spectroscopy. Journal of Physical Chemistry C, 2008, 112(16), 6499-6508. (JCR三区, IF: 3.2)

专利

1.      一种无标记荧光检测FEN1活性的方法，专利号ZL202210142803.5

2.      一种检测基质金属蛋白酶的生物传感器及应用，专利号ZL202010848753.3

3.      一种同时检测多种DNA糖基化酶的荧光化学传感器、其检测方法及应用，专利号ZL202010067224.X

4.      一种检测甲基转移酶的单个量子点纳米传感器及其检测方法和应用，专利号ZL201911397409.0

5.      一种检测去乙酰化酶用纳米传感器及其检测方法和应用，专利号ZL202011209095.X

6.      一种检测DNA羟化酶TET1的纳米传感器及其检测方法和应用，专利号ZL202110261946.3

7.      一种检测5-羟甲基胞嘧啶的化学发光生物传感器及其检测方法和应用，专利号ZL202011195224.4

8.      一种同时检测多种长链非编码RNA的复合物及方法，专利号ZL202110069247.9

9.      一步法荧光检测体系及DNA糖基化酶活性的检测方法和应用，专利号ZL202110361586.4

10.   基于单量子点的纳米传感器检测人烷基糖基化酶的方法，专利号ZL201910199129.2

11.   恒温扩增与量子点荧光共振能量转移联用用于同时检测多种MicroRNAs的方法，专利号ZL201810300609.9

12.   一种在单分子水平同时检测多种DNA糖基化酶的荧光化学传感器及其检测方法和应用，专利号ZL201711027692.9

13.   一种量子产率的测试方法，专利号ZL201310120635.0

14.   多组分生物标识物的检测方法，专利号ZL201010167849.X