主持

1.国家自然科学基金面上项目，催化自组装量子点生物传感器的构筑及其在乳腺癌诊断中的应用研究

2.国家自然科学基金青年基金，基于核酸恒温扩增技术的组蛋白修饰超灵敏定量分析方法研究

3.山东省自然科学基金青年基金，组蛋白修饰酶的超灵敏检测及其在癌症早期诊断中的应用

参与

1.国家自然科学基金重点项目，靶向肺癌循环肿瘤细胞的单分子检测技术研究

2.国家自然科学基金面上项目，表面增强拉曼光谱及成像技术在肿瘤细胞检测中的应用研究

3.国家自然科学基金青年基金，稀有核酸碱基的光化学反应动力学机理研究

4.国家自然科学基金青年基金，基于等温扩增的脱氧核酶荧光生物传感器检测肿瘤标志物技术的研究

5.国家自然科学基金青年基金，DNA糖基化酶的单分子检测及其在癌症早期诊断中的应用研究

一作及通讯论文

69. Weijie Tong,† Yun Han,† Tao Wang,† Jiayi Wan, Fei Ma,* Chun-yang Zhang.* Bidirectional polymerization-transcription amplification-encoded dual-color fluorescent biosensor for label-free and primer-free detection of multiple piRNAs. Analytical Chemistry, 2024, 96, 38, 15484–15492.

68. Huimin Yuan,† Jinping Hu,† Qi-qin Ge,† Wen-jing Liu, Fei Ma,* Chun-yang Zhang.* Construction of a spatial-confined self-stacking catalytic circuit for rapid and sensitive imaging of piwi-interacting RNA in living cells. Nano Letters, 2024, 24, 28, 8732–8740.

67. Qian Zhang, Huan Liu, Qinfeng Xu, Hao Liu,Yun Han, Dong-Ling Li, Fei Ma,* Chun-yang Zhang.* Construction of a 3D quantum dot nanoassembly with two-step FRET for one-step sensing of human telomerase RNA in breast cancer cells and tissues. Analytical Chemistry, 2024, 96, 19,7738–7746.

66. Yun Han, Qinfeng Xu, Hao Liu, Fei Ma,* Chun-Yang Zhang.* Advance in intracellular telomerase imaging for cancer diagnosis and therapy. Coordination Chemistry Reviews, 2024, 509, 215801.

65. Yun Han, Dong-ling Li, Qian Han, Fei Ma,* Chun-yang Zhang.* Integration of demethylation-activated DNAzyme with a single quantum dot nanosensor for sensitive detection of O6-methylguanine DNA methyltransferase in breast tissues. Analytical Chemistry, 2024, 96, 11, 4487–4494.

64. Yun Han,† Dongming Yang,† Su Jiang,† Shulin Zhao,* Fei Ma,* Chun-yang Zhang.* Recent advance in optical single-molecule detection of methylation modification and methyl-modifying enzymes. Trends in Analytical Chemistry, 2024, 172, 117553.

63. Qi-qin Ge, Qian Han, Yun Han, Fei Ma,* Chen-zhong Li,* Chun-yang Zhang.* A multi-cycle signal amplification-mediated single quantum dotnanosensor for PIWI-interacting RNA detection. Chemical Communications, 2024,60, 408-411.

62.Meng Liu,† Nan Zhong,† Lingfei Zhang,† Qian Zhang,† Xiaorui Tian, Fei Ma,* Chun-yang Zhang.* Single probe-based catalytic quantum dot FRET nanosensor for human alkyladenine DNA glycosylase detection, Talanta, 2024, 266, 125089.

61.Jinping Hu,† Ya-ting Zhang,† Yun Han, Fei Ma,* Chen-zhong Li,* Lin Cui,* Chun-yang Zhang.* Methylation-powered assembly of a single quantum dot-based FRET nanosensor for antibody-free and enzyme-free monitoring of locus-specific N6-methyladenosine in clinical tissues. Analytical Chemistry, 2023, 95, 48, 17945–17953.

60.Wen-jing Liu,† Hai-juan Li,† Xiaoran Zou,† Qian Liu, Fei Ma,* Chun-yang Zhang.* Deamination-triggered exponential signal amplification for chemiluminescent detection of cytosine deaminase at the single-cell level, Chemical Communications, 2023, 59, 11807-11810.

59.Li-juan Wang, Qian Liu, Fei Ma,* Chun-yang Zhang.* Target-responsive DNAzyme probes for luminescence detection and imaging of DNA-modifying enzymes,Trends in Analytical Chemistry, 2023, 167, 117270.

58.Na Li,† Lingfei Zhang,† Hao Liu,Qinfeng Xu, Fei Ma,* Chun-yang Zhang.* Label-free and sensitive detection of N6-methyladenosine demethylase activity in crude cell extracts and clinical cancer tissues based on demethylation-triggered exponential signal amplification. Analytica Chimica Acta, 2023, 1278, 341705.

57.Fei Ma,† Xiao-di Yu,† Wenjing Liu,† Hao Liu, Qinfeng Xu,* Chun-yang Zhang.* Dual signal amplification-integrated single-molecule biosensing of flap endonuclease1 in breast cancer tissues. Sensors and Actuators B: Chemical, 2023, 394,134383.

56.Ning-ning Zhao,† Xiaorui Tian,† Fei Ma,* Chun-yang Zhang.* CRISPR/Cas12a-enhanced single-molecule counting for sensitive detection of flap endonuclease 1 activity at the single-cell level. Chemical Communications, 2023, 59, 4939-4942.

55.Fei Ma,*† Xinyi Zhang.† Single-molecule/particle counting for microRNA detection and imaging. Trends in Analytical Chemistry, 2023, 164, 117085.

54.Wen-jing Liu,† Xinyi Zhang,† Fei Ma,* Chun-yang Zhang.* Recent advance in nucleic acid amplification-integrated methods for DNA methyltransferase assay. Trends in Analytical Chemistry, 2023, 160, 116998.

53.Zi-wei Han, Fei Ma,* Chun-yang Zhang.* Methylation-sensitive transcription-enhanced single-molecule biosensing of DNA methylation in cancer cells and tissues. Analytica Chimica Acta, 2023, 1251, 340996.

52.Qian Zhang,† Xinyi Zhang,† Xiaoran Zou, Fei Ma,* Chun-Yang Zhang.* Crispr/Cas-based microRNA biosensors. Chemistry-A European Journal, 2023, 29, e202203412.

51.Qian Zhang,† Zhen-zhen Yuan,† Xinyi Zhang,† Yan Zhang, Xiaoran Zou, Fei Ma,* Chun-yang Zhang.* Entropy-driven self-assembly of single quantum dot sensor for catalytic imaging of telomerase in living cells. Analytical Chemistry, 2022, 94, 51, 18092–18098.

50.Qian Zhang,† Ran Zhao,† Chen-chen Li,† Yan Zhang, Chunying Tang, Xiliang Luo,* Fei Ma,* Chun-yang Zhang.* Construction of an entropy-driven dumbbell-type DNAzyme assembly circuit for lighting up uracil-DNA glycosylase in living cells. Analytical Chemistry, 2022, 94, 40, 13978–13986.

49.Fei Ma,† Ya-zhen Liu,† Meng Liu,† Jian-Ge Qiu,* Chun-yang Zhang.* Transcriptionally amplified synthesis of fluorogenic RNA aptamers for label-free DNA glycosylase assay. Chemical Communications, 2022, 58, 10229-10232.

48.Su Jiang,† Xinyi Zhang,† Dong-ling Li, Ting-ting Wang, Fei Ma,* Chun-yang Zhang.* Construction of a gold nanoparticle-based single-molecule biosensor for simple and sensitive detection of Argonaute 2 activity. Journal of Materials Chemistry B, 2022, 10, 5594-5601.

47.Qian Zhang,† Xinyi Zhang,† Fei Ma,* Chun-yang Zhang.* Advances in quantum dot-based biosensors for DNA-modifying enzymes assay. Coordination Chemistry Reviews, 2022, 469, 214674.

46.Qian Zhang,† Chen-chen Li,† Fei Ma,* Xiliang Luo,* Chun-yang Zhang.* Catalytic single-molecule Förster resonance energy transfer biosensor for uracil-DNA glycosylase detection and cellular imaging. Biosensors and Bioelectronics, 2022, 213, 114447.

45.Qian Zhang,† Yun Han,† Chen-chen Li,† Xiaoran Zou, Fei Ma,* Chun-yang Zhang.* Construction of a dual-functional dumbbell probe-based fluorescent biosensor for cascade amplification detection of miRNAs in lung cancer cells and tissues. Chemical Communications, 2022, 58, 5538-5541.

44.Fei Ma,† Ning-ning Zhao,† Meng Liu,† Qinfeng Xu,* Chun-yang Zhang.* Single-molecule biosensing of alkaline phosphatase in cells and serum based ondephosphorylation-triggered catalytic assembly and disassembly of the fluorescent DNA chain. Analytical Chemistry, 2022, 94, 15, 6004–6010.

43.Meng Liu,† Rui Xu,† Wenjing Liu,† Jian-Ge Qiu,* Yan Wang,* Fei Ma,* Chun-yang Zhang.* Integration of exonuclease III-powered three-dimensional DNA walker with single-molecule detection for multiple initiator caspases assay. Chemical Science, 2021, 12, 15645-15654.

42.Fei Ma,† Qi Wang,† Qinfeng Xu,† Chun-yang Zhang.* Self-assembly of super quenched gold nanoparticle nanosensors for lighting up BACE-1 in live cells. Analytical Chemistry, 2021, 93, 45, 15124–15132.

41.Fei Ma,† Chen-Chen Li,† Chun-Yang Zhang.* Nucleic acid amplification-integrated single-molecule fluorescence imaging for in vitro and in vivo biosensing. Chemical Communications, 2021, 57, 13415-13428. (outside front cover)

40.Yan Zhang,† Yun Han,† Xiaoran Zou,† Qinfeng Xu, Fei Ma,* Chun-yang Zhang.* Construction of a damage site-specific fluorescent biosensor for single-molecule detection of DNA damage. Talanta, 2021, 235, 122809.

39.Meng Liu,† Jian-Ge Qiu,† Fei Ma,* Chun-yang Zhang.* Advances in single-molecule fluorescent nanosensors. WIREs Nanomedicine & Nanobiotechnology, 2021, e1716.

38.Yan Zhang,† Jinping Hu,† Xiaoran Zou,† Fei Ma,* Jian-Ge Qiu,* Chun-yang Zhang.* Integration of single-molecule detection with endonuclease IV-assisted signal amplification for sensitive DNA methylation assay. Chemical Communications, 2021, 57(16), 2073-2076.

37.Su Jiang,† Meng Liu,† Wei Tantai, Qinfeng Xu, Xiaoran Zou, Fei Ma,* Chun-yangZhang.* Aptamer-mediated rolling circle amplification for label-free and sensitive detection of histone acetyltransferase activity. Chemical Communications, 2021, 57(16), 2041-2044.

36.Ya-ru Meng,† Dandan Zhang,† Xiaoran Zou, Fei Ma,* Qi Kang,* Chun-yang Zhang.* A trifunctional split dumbbell probe coupled with ligation-triggered isothermal rolling circle amplification for label-free and sensitive detection of nicotinamide adenine dinucleotide. Talanta, 2021, 224, 121962.

35.Qian Zhang,† Yanxia Wu,† Qinfeng Xu, Fei Ma,* Chun-yang Zhang.* Recent advances in biosensors for in vitro detection and in vivo imaging of DNA methylation. Biosensors and Bioelectronics, 2021, 171, 112712. (ESI highly cited paper)

34.Fei Ma,† Qian Zhang,† Chun-yang Zhang.* Nanomaterial-based biosensors for DNA methyltransferase assay. Journal of Materials Chemistry B, 2020, 8(16),3488-3501.

33.Meng Liu,† Di Zhang,† Xuechong Zhang, Qinfeng Xu, Fei Ma,* Chun-yang Zhang.* Label-free and amplified detection of apoptosis-associated caspase using branched rolling circle amplification. Chemical Communications, 2020, 56(39),5243-5246.

32.Fei Ma,† Su Jiang,† Chun-yang Zhang.* SiRNA-directed self-assembled quantum dot biosensor for simultaneous detection of multiple microRNAs at the single-particle level. Biosensors and Bioelectronics, 2020, 157, 112177.

31.Meng Liu,† Chen-chen Li,† Xiliang Luo,* Fei Ma,* Chun-yang Zhang.* 5-Hydroxymethylcytosine glucosylation-triggered helicase-dependent amplification-based fluorescent biosensor for sensitive detection of β-glucosyltransferase with zero background signal. Analytical Chemistry, 2020, 92(24), 16307–16313.

30.Lijian Zhang,† Fei Ma,† Ao Qi, Lulu Liu, Junjie Zhang, Simin Xu, Qisheng Zhong, Yusen Chen,* Chun-yang Zhang,* Chun Cai.* Integration of ultra-high-pressure liquid chromatography-tandem mass spectrometry with machine learning for identifying fatty acid metabolite biomarkers of ischemic stroke. Chemical Communications,2020, 56(49), 6656-6659.

29.Fei Ma,† Qian Zhang,† Chun-yang Zhang.* Catalytic self-assembly of quantum dot-based microRNA nanosensor directed by toehold-mediated strand displacement cascade. Nano Letters, 2019, 19(9), 6370-6376.

28.Fei Ma,† Shu-hua Wei,† Chun-yang Zhang.* Construction of a robust entropy-driven DNA nanomachine for single-molecule detection of rare cancer cells. Analytical Chemistry, 2019, 91(12), 12, 7505-7509.

27.Fei Ma,† Meng Liu,† Chun-yang Zhang.* Ligase amplification reaction-catalyzed assembly of a single quantum dot-based nanosensor for sensitive detection of alkaline phosphatase. Chemical Communications, 2019, 55(61), 8963-8966.

26.Fei Ma,† Ting-ting Wang,† Longhe Jiang,† Chun-yang Zhang.* Ultrasensitive detection of telomerase activity in lung cancer cells with quencher-free molecular beacon-assisted quadratic signal amplification. Analytica Chimica Acta,2019,1053,122-130.

25.Fei Ma,† Su Jiang,† Chun-yang Zhang.* Recent advances in histone modification and histone modifying enzyme assays. Expert Review of Molecular Diagnostics, 2019,19(1), 27-36.

24.Fei Ma,† Huan Liu,† Chen-chen Li,† Chun-yang Zhang.* A simple and isothermal ligase-based amplification approach based on a ligation-activated cleavage reaction. Chemical Communications, 2018,54(89), 12638-12641.

23.Fei Ma,† Shu-hua Wei,† Junhong Leng,† Bo Tang,* Chun-yang Zhang.* A simple "mix-and-detection" method for the sensitive detection of telomerase from cancer cells under absolutely isothermal conditions. Chemical Communications, 2018, 54(20), 2483-2486.

22.Fei Ma,† Wen-jing Liu,† Li Liang,† Bo Tang,* Chun-yang Zhang.* Sensitive detection of alkaline phosphatase by dephosphorylation-initiated transcription reaction-mediated dual signal amplification. Chemical Communications, 2018,54(19), 2413-2416.

21.Fei Ma,† Chen-chen Li,† Chun-yang Zhang.* Development of quantum dot-based biosensors: principles and applications. Journal of Materials Chemistry B, 2018,6(39), 6173-6190. (2018 Journal of Materials Chemistry B HOT Papers)

20.Meng Liu,† Fei Ma,† Qianyi Zhang,† Chun-yang Zhang.* Label-free and ultrasensitive detection of polynucleotide kinase activity at the single-cell level. Chemical Communications, 2018, 54(13), 1583-1586.

19.Dandan Zhang,† Fei Ma,† Junhong Leng,† Chun-yang Zhang.* A dual signal amplification-assisted DNAzyme biosensor for ultrasensitive detection of Argonaute 2 activity. Chemical Communications, 2018, 54(97), 13678-13681.

18.Wen-jing Liu,† Qinfeng Xu,†* Fei Ma,† Chen-chen Li,† Chun-yang Zhang.* Exonuclease III-assisted multiple cycle amplification for the sensitive detection of DNA with zero background signal. Analyst, 2018, 143 (22), 5461-5466.

17.Fei Ma,† Meng Liu,† Bo Tang,* Chun-yang Zhang.* Sensitive quantification of microRNAs by isothermal helicase-dependent amplification. Analytical Chemistry, 2017, 89(11), 6182-6187.

16.Fei Ma,† Wen-jing Liu,† Bo Tang,* Chun-yang Zhang.* A single quantum dot-based nanosensor for the signal-on detection of DNA methyltransferase. Chemical Communications, 2017, 53 (51), 6868-6871.

15.Fei Ma,† Wen-jing Liu,† Qianyi Zhang,† Chun-yang Zhang.* Sensitive detection of microRNAs by duplex specific nuclease-assisted target recycling and pyrene excimer switching. Chemical Communications, 2017, 53 (76), 10596-10599.

14.Fei Ma,† Ming Ren,† Chun-yang Zhang.* Advances in single-particle detection for DNA sensing. Science China Chemistry, 2017, 60 (10), 1285-1292.

13.Yueying Li,† Xiaoran Zou,† Fei Ma,† Bo Tang,* Chun-yang Zhang.* Development of fluorescent methods for DNA methyltransferase assay. Methods and Applications in Fluorescence, 2017, 5(1), 012002.

12.Li-juan Wang,† Fei Ma,† Bo Tang,* Chun-yang Zhang.* Sensing telomerase: From in vitro detection to in vivo imaging. Chemical Science, 2017, 8(4), 2495-2502.

11.Qinfeng Xu,† Fei Ma,† Si-qiang Huang, Bo Tang,* Chun-yang Zhang*. Nucleic acid amplification-free bioluminescent detection of microRNAs with high sensitivity and accuracy based on controlled target degradation. Analytical Chemistry,2017,89(13), 7077-7083.

10.Dandan Zhang,† Fei Ma,† Qianyi Zhang,† Chun-yang Zhang.* Highly sensitive detection of epidermal growth factor receptor in lung cancer cells by aptamer-based target-/probe-mediated cyclic signal amplification. Chemical Communications, 2017, 53, 11496-11499.

9.Fei Ma,† Ying Li,† Bo Tang,* Chun-yang Zhang.* Fluorescent biosensors based on single-molecule counting. Accounts of Chemical Research, 2016, 49(9),1722-1730. (ESI highly cited paper)

8.Fei Ma,† Meng Liu,† Zi-yue Wang,† Chun-yang Zhang.* Multiplex detection of histone-modifying enzymes by total internal reflection fluorescence-based single-molecule detection. Chemical Communications, 2016, 52(6), 1218-1221.

7.Fei Ma, Chun-yang Zhang*. Histone modifying enzymes: novel disease biomarkers and assay development. Expert Review of Molecular Diagnostics, 2016, 16(3),297-306.

6.Li-juan Wang,† Fei Ma,† Bo Tang,* Chun-yang Zhang.* Base-excision-repair-induced construction of a single quantum-dot-based sensor for sensitive detection of DNA glycosylase activity. Analytical Chemistry, 2016, 88(15), 7523-7529.

5.Qinfeng Xu,† Si-qiang Huang,† Fei Ma,† Bo Tang,* Chun-yang Zhang.* Controllable mismatched ligation for bioluminescence screening of known and unknown mutations. Analytical Chemistry, 2016, 88(4), 2431-2439.

4.Yan Zhang,† Fei Ma,† Bo Tang,* Chun-yang Zhang.* Recent advances in transcription factor assays in vitro. Chemical Communications, 2016, 52, 4739-4748.

3.Fei Ma,† Yong Yang,† Chun-yang Zhang.* Ultrasensitive detection of transcription factors using transcription-mediated isothermally exponential amplification-induced chemiluminescence. Analytical Chemistry, 2014,86,6006-6011.

2.Fei Ma,† Lijuan Wang,† Jiale Li,† Muhammad Kaleem Samma, Yanjie Xie, Ren Wang, JinWang, Jing Zhang, Wenbiao Shen.* Interaction between HY1 and H2O2 in auxin-induced lateral root formation in Arabidopsis. Plant Molecular Biology,2014, 85, 49-61.

1.Lijuan Wang,† Fei Ma,† Sheng Xu, Tianqing Zheng, Ren Wang, Huiping Chen, Wenbiao Shen.* Cloning and characterization of a heme oxygenase-2 gene from rice (Oryzasativa L.), and its expression analysis in response to some abiotic stresses. Acta Physiologiae Plantarum, 2014, 36, 893-902.