主持项目：

1.国家自然科学基金面上项目（22174017）

2.国家自然科学基金青年基金（21705096）

3.山东省自然科学基金青年基金（ZR2016HQ07）

参与项目：

1.国家自然科学基金重点项目（21735003）

2.江苏省前沿技术研发计划（BF2024063）

3.国家自然科学基金面上项目（21575152）

4.国家自然科学基金青年基金（21603126）

5.国家自然科学基金青年基金（21605098）

6.国家自然科学基金青年基金（21705097）

2026

79. Ke Qin, Jiayin Zhao, Fei Ma,* Chun-yang Zhang.* Endogenous enzyme-activatable catalytic DNA nanodevice for cancer cell-selective piRNA imaging and regulation. Nano Today, 2026, 67, 102950

2025

78. Wen-jing Liu, Lu-yao Wang, Fei Ma,* Chun-yang Zhang.* MnO2 nanosponge-accelerated Cas12a trans-cleavage: Breaking the kinetic barrier for in vivo RNA imaging. Advanced Science, 2025, e11942

77. Wen-jing Liu, Junzhi Liu, Fei Ma,* Chun-yang Zhang.* CRISPR/Cas13a trans-cleavage-driven programmable autonomous synthesis of a functional G-quadruplex for ultrasensitive detection of circular RNAs with an improved signal-to-background ratio. Analytical Chemistry, 2025, 97, 37,20323–20332

75. Sirui Xiong, Yun Han, Mao-tao He, Fei Ma,* Chun-yang Zhang.* Engineering of a palindrome-crosslinked DNA nanoaggregate for rapid detection of circular RNA and precise identification of lung cancer. Biosensors and Bioelectronics, 2025, 284, 117564

74. Wen-jing Liu, Yun Han, Rui Song, Fei Ma,* Chun-yang Zhang.* Development of proximity-activated programmable multicomponent nucleic acid enzymes for simultaneous visualization of multiple mRNA splicing variants in living cells, Analytical Chemistry, 2025, 97, 14, 8098-8108

73. Yun Han, Weijie Tong, Fei Ma,* Chun-yang Zhang.* Recent advance in single-molecule detection and imaging. Trends in Analytical Chemistry, 2025,189, 118252

72. Ke-yuan Xia, Yun Han, Wanlong Xu, Fei Ma,* Chun-yang Zhang.* Construction of a methylation-directed duplex-specific nuclease amplification-based biosensor for simultaneous detection of locus-specific N6-methyladenosine at the single-molecule level.Sensors and Actuators B: Chemical, 2025, 432, 137494

71. Wen Li, Wen-Jing Liu, Jun Lu,* Fei Ma,* Chun-yang Zhang.* A programmable automatic cascade machinery for single-molecule profiling of multiple noncoding RNAs in breast tissues. Analytical Chemistry, 2025, 97, 7,4224-4232

2024

70. Na Li, Tao Wang, Qian Han, Ting-Ting Pan, Fei Ma,* Chun-yang Zhang.* Endogenous telomerase-activated fluorescent probes for specific detection and imaging of flap endonuclease 1 in cancer cells and tissues. Analytical Chemistry, 2024, 96, 48, 19147–19153

69. Weijie Tong, Yun Han, Tao Wang, Jiayi Wan, Fei Ma,* Chun-yang Zhang.* Bidirectional polymerization-transcription amplification-encoded dual-color fluorescent biosensor for label-free and primer-free detection of multiple piRNAs. Analytical Chemistry, 2024, 96, 38, 15484–15492.

68. Huimin Yuan, Jinping Hu, Qi-qin Ge, Wen-jing Liu, Fei Ma,* Chun-yang Zhang.* Construction of a spatial-confined self-stacking catalytic circuit for rapid and sensitive imaging of piwi-interacting RNA in living cells. Nano Letters, 2024, 24, 28, 8732–8740.

67. Qian Zhang, Huan Liu, Qinfeng Xu, Hao Liu,Yun Han, Dong-Ling Li, Fei Ma,* Chun-yang Zhang.* Construction of a 3D quantum dot nanoassembly with two-step FRET for one-step sensing of human telomerase RNA in breast cancer cells and tissues. Analytical Chemistry, 2024, 96, 19,7738–7746.

66. Yun Han, Qinfeng Xu, Hao Liu, Fei Ma,* Chun-Yang Zhang.* Advance in intracellular telomerase imaging for cancer diagnosis and therapy.Coordination Chemistry Reviews, 2024, 509, 215801.

65. Yun Han, Dong-ling Li, Qian Han, Fei Ma,* Chun-yang Zhang.* Integration of demethylation-activated DNAzyme with a single quantum dot nanosensor for sensitive detection of O6-methylguanine DNA methyltransferase in breast tissues. Analytical Chemistry, 2024, 96, 11, 4487–4494.

64. Yun Han, Dongming Yang, Su Jiang, Shulin Zhao,* Fei Ma,* Chun-yang Zhang.* Recent advance in optical single-molecule detection of methylation modification and methyl-modifying enzymes. Trends in Analytical Chemistry, 2024, 172, 117553.

63. Qi-qin Ge, Qian Han, Yun Han, Fei Ma,* Chen-zhong Li,* Chun-yang Zhang.* A multi-cycle signal amplification-mediated single quantum dot nanosensor for PIWI-interacting RNA detection. Chemical Communications,2024,60, 408-411.

62.Meng Liu, Nan Zhong, Lingfei Zhang, Qian Zhang, Xiaorui Tian, Fei Ma,* Chun-yang Zhang.* Single probe-based catalytic quantum dot FRET nanosensor for human alkyladenine DNA glycosylase detection, Talanta, 2024, 266, 125089.

2023

61.Jinping Hu, Ya-ting Zhang, Yun Han, Fei Ma,* Chen-zhong Li,* LinCui,* Chun-yang Zhang.* Methylation-powered assembly of a single quantum dot-based FRET nanosensor for antibody-free and enzyme-free monitoring of locus-specific N6-methyladenosine in clinical tissues. Analytical Chemistry,2023, 95, 48, 17945–17953.

60.Wen-jing Liu, Hai-juan Li, Xiaoran Zou, Qian Liu, Fei Ma,* Chun-yang Zhang.* Deamination-triggered exponential signal amplification for chemiluminescent detection of cytosine deaminase at the single-cell level,Chemical Communications, 2023, 59, 11807-11810.

59.Li-juan Wang, Qian Liu, Fei Ma,* Chun-yang Zhang.*Target-responsive DNAzyme probes for luminescence detection and imaging of DNA-modifying enzymes, Trends in Analytical Chemistry, 2023, 167, 117270.

58.Na Li, Lingfei Zhang, Hao Liu,Qinfeng Xu, Fei Ma,* Chun-yangZhang.* Label-free and sensitive detection of N6-methyladenosine demethylaseactivity in crude cell extracts and clinical cancer tissues based ondemethylation-triggered exponential signal amplification. Analytica ChimicaActa, 2023, 1278, 341705.

57.Fei Ma, Xiao-di Yu, Wenjing Liu, Hao Liu, Qinfeng Xu,* Chun-yangZhang.* Dual signal amplification-integrated single-molecule biosensing of flap endonuclease1 in breast cancer tissues. Sensors and Actuators B: Chemical,2023, 394,134383.

56.Ning-ning Zhao, Xiaorui Tian, Fei Ma,* Chun-yang Zhang.* CRISPR/Cas12a-enhanced single-molecule counting for sensitive detection of flap endonuclease 1 activity at the single-cell level. Chemical Communications,2023, 59, 4939-4942.

55.Fei Ma,* Xinyi Zhang. Single-molecule/particle counting formicroRNA detection and imaging. Trends in Analytical Chemistry, 2023, 164,117085.

54.Wen-jing Liu, Xinyi Zhang, Fei Ma,* Chun-yang Zhang.* Recent advance in nucleic acid amplification-integrated methods for DNA methyltransferase assay. Trends in Analytical Chemistry, 2023, 160, 116998.

53.Zi-wei Han, Fei Ma,* Chun-yang Zhang.* Methylation-sensitive transcription-enhanced single-molecule biosensing of DNA methylation in cancer cells and tissues. Analytica Chimica Acta, 2023, 1251, 340996.

52.Qian Zhang, Xinyi Zhang, Xiaoran Zou, Fei Ma,* Chun-Yang Zhang.*Crispr/Cas-based microRNA biosensors. Chemistry-A European Journal, 2023, 29,e202203412.

2022

51.Qian Zhang, Zhen-zhen Yuan, Xinyi Zhang, Yan Zhang, Xiaoran Zou,Fei Ma,* Chun-yang Zhang.* Entropy-driven self-assembly of single quantum dot sensor for catalytic imaging of telomerase in living cells. Analytical Chemistry, 2022, 94, 51, 18092–18098.

50.Qian Zhang, Ran Zhao, Chen-chen Li, Yan Zhang, Chunying Tang, Xiliang Luo,* Fei Ma,* Chun-yang Zhang.* Construction of an entropy-driven dumbbell-type DNAzyme assembly circuit for lighting up uracil-DNA glycosylase in living cells. Analytical Chemistry, 2022, 94, 40, 13978–13986.

49.Fei Ma, Ya-zhen Liu, Meng Liu, Jian-Ge Qiu,* Chun-yang Zhang.* Transcriptionally amplified synthesis of fluorogenic RNA aptamers for label-free DNA glycosylase assay. Chemical Communications, 2022, 58,10229-10232.

48.Su Jiang, Xinyi Zhang, Dong-ling Li, Ting-ting Wang, Fei Ma,* Chun-yang Zhang.* Construction of a gold nanoparticle-based single-molecule biosensor for simple and sensitive detection of Argonaute 2 activity. Journal of Materials Chemistry B, 2022, 10, 5594-5601.

47.Qian Zhang, Xinyi Zhang, Fei Ma,* Chun-yang Zhang.* Advances inquantum dot-based biosensors for DNA-modifying enzymes assay. Coordination Chemistry Reviews, 2022, 469, 214674.

46.Qian Zhang, Chen-chen Li, Fei Ma,* Xiliang Luo,* Chun-yangZhang.* Catalytic single-molecule Förster resonance energy transfer biosensorfor uracil-DNA glycosylase detection and cellular imaging. Biosensors and Bioelectronics, 2022, 213, 114447.

45.Qian Zhang, Yun Han, Chen-chen Li, Xiaoran Zou, Fei Ma,* Chun-yang Zhang.* Construction of a dual-functional dumbbell probe-based fluorescent biosensor for cascade amplification detection of miRNAs in lung cancer cells and tissues. Chemical Communications, 2022, 58, 5538-5541.

44.Fei Ma, Ning-ning Zhao, Meng Liu, Qinfeng Xu,* Chun-yang Zhang.* Single-molecule biosensing of alkaline phosphatase in cells and serum based on dephosphorylation-triggered catalytic assembly and disassembly of the fluorescent DNA chain. Analytical Chemistry, 2022, 94, 15, 6004–6010.

2021

43.Meng Liu, Rui Xu, Wenjing Liu, Jian-Ge Qiu,* Yan Wang,* Fei Ma,*Chun-yang Zhang.* Integration of exonuclease III-powered three-dimensional DNA walker with single-molecule detection for multiple initiator caspases assay. Chemical Science, 2021, 12, 15645-15654.

42.Fei Ma, Qi Wang, Qinfeng Xu, Chun-yang Zhang.* Self-assembly ofsuper quenched gold nanoparticle nanosensors for lighting up BACE-1 in livecells. Analytical Chemistry, 2021, 93, 45, 15124–15132.

41.Fei Ma, Chen-Chen Li, Chun-Yang Zhang.* Nucleic acid amplification-integrated single-molecule fluorescence imaging for in vitro and in vivo biosensing. Chemical Communications, 2021, 57, 13415-13428. (outside front cover)

40.Yan Zhang, Yun Han, Xiaoran Zou, Qinfeng Xu, Fei Ma,* Chun-yang Zhang.* Construction of a damage site-specific fluorescent biosensor for single-molecule detection of DNA damage. Talanta, 2021, 235, 122809.

39.Meng Liu, Jian-Ge Qiu, Fei Ma,* Chun-yang Zhang.* Advances in single-molecule fluorescent nanosensors. WIREs Nanomedicine &Nanobiotechnology, 2021, e1716.

38.Yan Zhang, Jinping Hu, Xiaoran Zou, Fei Ma,* Jian-Ge Qiu,*Chun-yang Zhang.* Integration of single-molecule detection with endonuclease IV-assisted signal amplification for sensitive DNA methylation assay. Chemical Communications, 2021, 57(16), 2073-2076.

37.Su Jiang, Meng Liu, Wei Tantai, Qinfeng Xu, Xiaoran Zou, Fei Ma,* Chun-yang Zhang.* Aptamer-mediated rolling circle amplification for label-free and sensitive detection of histone acetyltransferase activity. Chemical Communications, 2021, 57(16), 2041-2044.

36.Ya-ru Meng, Dandan Zhang, Xiaoran Zou, Fei Ma,* Qi Kang,*Chun-yang Zhang.* A trifunctional split dumbbell probe coupled with ligation-triggered isothermal rolling circle amplification for label-free and sensitive detection of nicotinamide adenine dinucleotide. Talanta, 2021, 224,121962.

2020

35.Qian Zhang, Yanxia Wu, Qinfeng Xu, Fei Ma,* Chun-yang Zhang.*Recent advances in biosensors for in vitro detection and in vivo imaging of DNAmethylation. Biosensors and Bioelectronics, 2021, 171, 112712. (ESI highlycited paper)

34.Fei Ma, Qian Zhang, Chun-yang Zhang.* Nanomaterial-based biosensors for DNA methyltransferase assay. Journal of Materials Chemistry B,2020, 8(16),3488-3501.

33.Meng Liu, Di Zhang, Xuechong Zhang, Qinfeng Xu, Fei Ma,*Chun-yang Zhang.* Label-free and amplified detection of apoptosis-associated caspase using branched rolling circle amplification. Chemical Communications,2020, 56(39),5243-5246.

32.Fei Ma, Su Jiang, Chun-yang Zhang.* SiRNA-directed self-assembled quantum dot biosensor for simultaneous detection of multiple microRNAs at the single-particle level. Biosensors and Bioelectronics, 2020, 157, 112177.

31.Meng Liu, Chen-chen Li, Xiliang Luo,* Fei Ma,* Chun-yang Zhang.* 5-Hydroxymethylcytosine glucosylation-triggered helicase-dependent amplification-based fluorescent biosensor for sensitive detection of β-glucosyltransferase with zero background signal. Analytical Chemistry, 2020,92(24), 16307–16313.

30.Lijian Zhang, Fei Ma, Ao Qi, Lulu Liu, Junjie Zhang, Simin Xu,Qisheng Zhong, Yusen Chen,* Chun-yang Zhang,* Chun Cai.* Integration of ultra-high-pressure liquid chromatography-tandem mass spectrometry with machine learning for identifying fatty acid metabolite biomarkers of ischemic stroke.Chemical Communications,2020, 56(49), 6656-6659.

2019

29.Fei Ma, Qian Zhang, Chun-yang Zhang.* Catalytic self-assembly of quantum dot-based microRNA nanosensor directed by toehold-mediated strand displacement cascade. Nano Letters, 2019, 19(9), 6370-6376.

28.Fei Ma, Shu-hua Wei, Chun-yang Zhang.* Construction of a robust entropy-driven DNA nanomachine for single-molecule detection of rare cancer cells. Analytical Chemistry, 2019, 91(12), 12, 7505-7509.

27.Fei Ma, Meng Liu, Chun-yang Zhang.* Ligase amplification reaction-catalyzed assembly of a single quantum dot-based nanosensor for sensitive detection of alkaline phosphatase. Chemical Communications, 2019,55(61), 8963-8966.

26.Fei Ma, Ting-ting Wang, Longhe Jiang, Chun-yang Zhang.* Ultrasensitive detection of telomerase activity in lung cancer cells with quencher-free molecular beacon-assisted quadratic signal amplification. Analytica Chimica Acta,2019,1053,122-130.

25.Fei Ma, Su Jiang, Chun-yang Zhang.* Recent advances in histone modification and histone modifying enzyme assays. Expert Review of Molecular Diagnostics, 2019,19(1), 27-36.

2018

24.Fei Ma, Huan Liu, Chen-chen Li, Chun-yang Zhang.* A simple and isothermal ligase-based amplification approach based on a ligation-activated cleavage reaction. Chemical Communications, 2018,54(89), 12638-12641.

23.Fei Ma, Shu-hua Wei, Junhong Leng, Bo Tang,* Chun-yang Zhang.* A simple "mix-and-detection" method for the sensitive detection of telomerase from cancer cells under absolutely isothermal conditions. Chemical Communications, 2018, 54(20), 2483-2486.

22.Fei Ma, Wen-jing Liu, Li Liang, Bo Tang,* Chun-yang Zhang.* Sensitive detection of alkaline phosphatase by dephosphorylation-initiated transcription reaction-mediated dual signal amplification. Chemical Communications, 2018,54(19), 2413-2416.

21.Fei Ma, Chen-chen Li, Chun-yang Zhang.* Development of quantum dot-based biosensors: principles and applications. Journal of Materials Chemistry B, 2018,6(39), 6173-6190. (2018 Journal of Materials Chemistry B HOT Papers)

20.Meng Liu, Fei Ma, Qianyi Zhang, Chun-yang Zhang.* Label-free and ultrasensitive detection of polynucleotide kinase activity at the single-cell level. Chemical Communications, 2018, 54(13), 1583-1586.

19.Dandan Zhang, Fei Ma, Junhong Leng, Chun-yang Zhang.* A dual signal amplification-assisted DNAzyme biosensor for ultrasensitive detection of Argonaute 2 activity. Chemical Communications, 2018, 54(97), 13678-13681.

18.Wen-jing Liu, Qinfeng Xu,* Fei Ma, Chen-chen Li, Chun-yang Zhang.* Exonuclease III-assisted multiple cycle amplification for the sensitive detection of DNA with zero background signal. Analyst, 2018, 143 (22),5461-5466.

2017

17.Fei Ma, Meng Liu, Bo Tang,* Chun-yang Zhang.* Sensitive quantification of microRNAs by isothermal helicase-dependent amplification. Analytical Chemistry, 2017, 89(11), 6182-6187.

16.Fei Ma, Wen-jing Liu, Bo Tang,* Chun-yang Zhang.* A single quantum dot-based nanosensor for the signal-on detection of DNA methyltransferase. Chemical Communications, 2017, 53 (51), 6868-6871.

15.Fei Ma, Wen-jing Liu, Qianyi Zhang, Chun-yang Zhang.* Sensitive detection of microRNAs by duplex specific nuclease-assisted target recycling and pyrene excimer switching. Chemical Communications, 2017, 53 (76),10596-10599.

14.Fei Ma, Ming Ren, Chun-yang Zhang.* Advances in single-particle detection for DNA sensing. Science China Chemistry, 2017, 60 (10), 1285-1292.

13.Yueying Li, Xiaoran Zou, Fei Ma, Bo Tang,* Chun-yang Zhang.* Development of fluorescent methods for DNA methyltransferase assay. Methods and Applications in Fluorescence, 2017, 5(1), 012002.

12.Li-juan Wang, Fei Ma, Bo Tang,* Chun-yang Zhang.* Sensing telomerase: From in vitro detection to in vivo imaging. Chemical Science, 2017,8(4), 2495-2502.

11.Qinfeng Xu, Fei Ma, Si-qiang Huang, Bo Tang,* Chun-yang Zhang*. Nucleic acid amplification-free bioluminescent detection of microRNAs with high sensitivity and accuracy based on controlled target degradation. Analytical Chemistry,2017,89(13), 7077-7083.

10.Dandan Zhang, Fei Ma, Qianyi Zhang, Chun-yang Zhang.* Highly sensitive detection of epidermal growth factor receptor in lung cancer cells by aptamer-based target-/probe-mediated cyclic signal amplification. Chemical Communications, 2017, 53, 11496-11499.

2016

9.Fei Ma, Ying Li, Bo Tang,* Chun-yang Zhang.* Fluorescent biosensors based on single-molecule counting. Accounts of Chemical Research,2016, 49(9),1722-1730. (ESI highly cited paper)

8.Fei Ma, Meng Liu, Zi-yue Wang, Chun-yang Zhang.* Multiplex detection of histone-modifying enzymes by total internal reflection fluorescence-based single-molecule detection. Chemical Communications, 2016,52(6), 1218-1221.

7.Fei Ma, Chun-yang Zhang.* Histone modifying enzymes: novel diseasebiomarkers and assay development. Expert Review of Molecular Diagnostics, 2016,16(3),297-306.

6.Li-juan Wang, Fei Ma, Bo Tang,* Chun-yang Zhang.* Base-excision-repair-induced construction of a single quantum-dot-based sensor for sensitive detection of DNA glycosylase activity. Analytical Chemistry,2016, 88(15), 7523-7529.

5.Qinfeng Xu, Si-qiang Huang, Fei Ma, Bo Tang,* Chun-yang Zhang.* Controllable mismatched ligation for bioluminescence screening of known and unknown mutations. Analytical Chemistry, 2016, 88(4), 2431-2439.

4.Yan Zhang, Fei Ma, Bo Tang,* Chun-yang Zhang.* Recent advances intranscription factor assays in vitro. Chemical Communications, 2016, 52,4739-4748.

3.Fei Ma, Yong Yang, Chun-yang Zhang.* Ultrasensitive detection of transcription factors using transcription-mediated isothermally exponential amplification-induced chemiluminescence. Analytical Chemistry,2014,86,6006-6011.

2014

2.Fei Ma, Lijuan Wang, Jiale Li, Muhammad Kaleem Samma, Yanjie Xie, Ren Wang, JinWang, Jing Zhang, Wenbiao Shen.* Interaction between HY1 and H2O2 in auxin-induced lateral root formation in Arabidopsis. Plant Molecular Biology, 2014, 85, 49-61.

1.Lijuan Wang, Fei Ma, Sheng Xu, Tianqing Zheng, Ren Wang, Huiping Chen, Wenbiao Shen.* Cloning and characterization of a heme oxygenase-2 gene from rice (Oryzasativa L.), and its expression analysis in response to some abiotic stresses. Acta Physiologiae Plantarum, 2014, 36, 893-902.