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儿童智力发育迟缓和老年人群中的神经退行性疾病是常见多发的神经系统疾病,目前人们对其复杂的发病机理知之甚少,缺乏有效的治疗手段,是生物医学领域里未解的难题。对神经发育,突触形成和维持,神经递质的传递和神经环路形成过程中关键分子及分子事件的研究是解开难题的核心。我们主要运用生物化学与分子生物学,细胞生物学,结构生物学,生物信息学和遗传学等方法与手段,通过对细胞内蛋白质的表达调控,蛋白质之间的相互作用,蛋白质和RNA等生物大分子在细胞内的转运以及相应细胞生理生化功能的研究,来揭示神经发育与退化过程的分子机制,探讨智力发育迟缓和神经退行性性疾病的发病机理,寻找新的治疗手段。
1. Gu,Q; Wu, J; Tian, Y; Cheng, S; Zhang,Z. C*; Han, J.*; Galphaqsplice variants mediate phototransduction,rhodopsin synthesis, and retinal integrity in Drosophila, J Biol Chem,2020, 295. (cover paper)
2. Liu, X; Dou, L. X; Han, J; Zhang, Z. C.*;The Renpenningsyndrome-associated protein PQBP1 facilitates the nuclear import of splicingfactor TXNL4A through the karyopherinbeta2 receptor, JBiol Chem,2020, 295(13): 4093-4100. (cover paper)
3. Mu Y.; Tian Y.; Zhang Z.C.*;Han J.*; Metallophosphoesteraseregulates light-induced rhodopsin endocytosis by promoting an associationbetween arrestinand the adaptor protein AP2 , JBiol Chem,2019, 294(35): 12892-12900.
4. GuoL.; Kim H.J.; Wang H.; Monaghan J.; FreyermuthF.; Sung J.C.;O'DonovanK.; Fare C.M.; Diaz Z.; Singh N.; Zhang Z.C.;Coughlin M.; Sweeny E.A.; DeSantis M.E.; JackrelM.E.; RodellC.B.; Burdick J.A.; King O.D.; GitlerA.D.; LagierT.C.; Pandey U.B.; Chook Y.M.; Taylor J.P.*; Shorter J.*; Nuclear-ImportReceptors Reverse Aberrant Phase Transitions of RNA-Binding Proteins withPrion-like Domains , Cell,2018, 173(3): 677-692.
5. Zhang X.-Y.; Qi J.; Shen Y.-Q.; LiuX.; Liu A.; Zhou Z.; Han J.; ZhangZ.C.*; Mutations of PQBP1 in Renpenningsyndrome promote ubiquitin-mediated degradation of FMRP and cause synapticdysfunction, HumMol Genetics,2017, 26(5): 955-968.
6. TianY.; ZhangZ.C.; Han J.*; Drosophila Studies onAutism Spectrum Disorders , Neurosci Bull,2017, 33(6):737-746.
7. Li Q.; Wang X.; Qi J.; JinX.; Tong H.; Zhou Z.; ZhangZ.C.; Han J.*; Fbxl4 Serves as a ClockOutput Molecule That Regulates Sleep Through Promotion of Rhythmic Degradationof the GABA A Receptor, Curr Biol,2017, 27(23):3616-3625.
8.Tong H.; Li Q.; Zhang Z.C.;Li Y.; Han J.*; Neurexinregulates nighttime sleep by modulating synaptic transmission , Scientific Rep,2016, 6(1): 38246-38246.
9. Wei J.-H.*; Zhang Z.C.;Wynn R. M.; SeemannJ.*; GM130 Regulates Golgi-Derived Spindle Assembly by Activating TPX2 andCapturing Microtubules, Cell,2015, 162(2): 287-299.
10. Wan D.#; Zhang Z. C.#;Zhang X.; Li Q.; Han J.*; X-chromosome-linked intellectual disability proteinPQBP1 associates with and regulates the translation of specific mRNAs, Hum Mol Genetics,2015, 24(16):4599-4614.
11. EtchinJ.; Sun Q.; KentsisA.; Farmer A.; ZhangZ. C.; SandaT.;MansourM. R.; BarceloC.; McCauley D.; Kauffman M.; ShachamS.; Christie A.L.; Kung A. L.; RodigS. J.; Chook Y. M.*; Look A. T.*; Antileukemicactivity of nuclear export inhibitors that spare normal hematopoietic cells, Leukemia,2013, 27(1): 66-74.
12. Li Y.#; Zhou Z.#; Zhang X.;Tong H.; Li P.; ZhangZ. C.; JiaZ.; XieW.*; Han J.*; Drosophila neuroligin4 regulates sleep through modulating GABA transmission, J Neurosic,2013,33(39): 15545-54.
13. Tian Y.; Li T.; Sun M.; Wan D.;Li Q.; Li P.; ZhangZ. C.; Han J.*; XieW.*; Neurexinregulates visual function via mediating retinoid transport to promote rhodopsinmaturation , Neuron,2013, 77(2): 311-22.
14. Zhang Z. C.;Chook Y. M.*; Structural and energetic basis of ALS-causing mutations in theatypical proline-tyrosinenuclear localization signal of the Fused in Sarcoma protein (FUS), PNAS,2012, 109(30): 12017-21.
15. Zhang Z. C.;SatterlyN.; FontouraB. M.; Chook Y. M.*; Evolutionary development of redundant nuclear localizationsignals in the mRNA export factor NXF1, Mol Biol Cell,2011, 22(23): 4657-68.
16. CansizogluA. E.; Lee B. J.; ZhangZ. C.; FontouraB. M.; Chook Y. M.*; Structure-based design of a pathway-specific nuclearimport inhibitor, NatStruct & Mol Biol,2007, 14(5): 452-4.
17. Qian F.; Li Y. P.; Sheng X.; Zhang Z. C.;Song R.; Dong W.; Cao S. X.; Hua Z. C.; Xu Q.*; PRL-3 siRNA inhibits themetastasis of B16-BL6 mouse melanoma cells in vitro and in vivo, Mol Medicine,2007, 13(3-4): 151-9.
18. Lee B. J.; CansizogluA. E.; SuelK. E.; Louis T. H.; ZhangZ.C.; Chook Y. M.*; Rules for nuclearlocalization sequence recognition by karyopherinbeta 2, Cell,2006, 126(3): 543-58.
19. Qian F.#; Zhang Z. C.#;Wu X. F.; Li Y. P.; Xu Q.*; Interaction between integrin alpha(5) andfibronectin is required for metastasis of B16F10 melanoma cells, Biochem Biophys Res Commun,2005, 333(4): 1269-75.
20.ZhangZ.C.; Chook Y.M.*; Structural Analysis of Karyopherin-mediatednucleocytoplasmic transport, LandesBioscienc,3千字,2009. (学术专著)
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