主要从事感音性耳聋的致病机制研究，具体研究方向：1）老年性耳聋的发病机制；2）遗传性耳聋的致聋机理；3）听觉毛细胞发育关键调控因子的功能研究以及毛细胞再生研究。

 作为课题三负责人（项目骨干）参加国家重点研发计划干细胞及转化研究青年项目一项，主持国家重点研发计划重点专项子课题一项，主持国家自然科学基金青年项目一项，主持中国博士后65批基金一项，主持山东省博士后自主创新项目一项。授权发明专利两项。

国家重点研发计划干细胞及转化青年项目 “肝脏干/祖细胞标记物鉴定及3D肝脏微器官构建” 课题三负责人  2019YFAO111400  120万

国家重点研发计划重点专项“人类精子成熟关键分子的作用机制和临床转化研究” 子课题负责人  2018YFC1003600  50万

国家自然科学基金青年基金 “mTOR信号通路在耳蜗毛细胞发育和存活中的调控作用及其机制研究”  81900937   23万

中国博士后基金第65批基金 “DFNB110基因缺失对内耳发育的影响及其致聋机制研究”2019M652400  8万

山东省高等学校青年创新团队  铁死亡通路在顺铂诱发耳毒性中的作用研究    45万

山东省博士后自主创新基金一等资助 DFNB110基因缺失致聋机制研究  10万

2020年中央高校基础基础科研业务费 Pex5内耳特异性敲除小鼠的的构建与分析  2242020K40216  20万

2019年中央高校基础基础科研业务费  mTOR 信号通路在听觉系统中的作用机制研究 8.8万

Dock4 Is Required for the Maintenance of Cochlear Hair Cells and Hearing Function. Guodong Hong# , Xiaolong Fu# , Jieyu Qi# , Buwei Shao#, Xuan Han , Yuan Fang , Shuang Liu , Cheng Cheng , Chengwen Zhu , Junyan Gao , Xia Gao, Jie Chen*, Ming Xia*, Wei Xiong*, Renjie Chai*. Fundamental Research (并列一作）

 Gstm1/Gstt1 is essential for reducing cisplatin ototoxicity in CBA/CaJ mice. Peipei Li#, Ziyi Liu#, Jinpeng Wang#, , Xiuli Bi , Yu Xiao , Ruifeng Qiao , Xuanchen Zhou , Siwei Guo , Peifeng Wan , Miao Chang , Guodong Hong , Zhangsuo Liu , Xia Ming , Jiangang Gao*, Xiaolong Fu*  the FASEB journal (最终通讯）IF=5.191

 Single-Cell Transcriptomic Atlas of Mouse Cochlear Aging. Guoqiang Sun#, Yandong Zheng#, Xiaolong Fu# Weiqi Zhang#, Shuai Ma, Xiaojuan He, Qiaoran Wang, Zhejun Ji, Fang Cheng, Kaowen Yan, Juan Carlos Izpisua Belmonte , Jing Qu*, Si Wang*, Renjie Chai3*, Guang-Hui Liu*. Cell Discovery （并列一作） IF=14.281

 SMPX deficiency causes stereocilia degeneration and progressive hearing loss in C57BL/6J mice. Hailong Tu, Aizhen Zhang, Xiaolong Fu*, Xiaohui Bai, Haibo Wang*, Jiangang Gao*. Front. Cell Dev. Biol  10.3389/fcell.2021.750023  (并列通讯）IF=6.684

 Xiaolong Fu, Peipei Li , Linqing Zhang ,Yuning Song，Yachun An，Aizhen Zhang，Wenwen Liu，Chao Ye, Yuan Zhang, Xiaoyang Sun，Renjie Chai *，Haibo Wang *，Jiangang Gao *Activation of Rictor/mTORC2 signaling acts as a new pivotal strategy to protect against sensorineural hearing loss  PNAS  10.1073/pnas.2107357119 2022 （第一作者）IF=11.205 

 Yuan Zhang#, Xiaolong Fu#, Yiyuan Li# , Guodong Hong, Jin Jin*, Renjie Chai*  Lack of FAM73a and FAM73b causes deafness by promoting macrophage-mediated hair cell death  Cellular and Molecular Life Sciences   （并列一作） IF=9.261

Yuan Zhang#, Yiyuan Li#, Xiaolong Fu#, Pengjun Wang, Qin Wang, Wei Meng*, Tian Wang*, Jianming Yang*, Renjie Chai* The detrimental and beneficial functions of macrophages after cochlear injury  Front. Cell Dev. Biol. https://doi.org/10.3389/fcell.2021.631904（并列一作） IF=6.684

Xiaolong Fu#, Peifeng Wan#, Peipei Li#, Jinpeng Wang, Siwei Guo, Yuan Zhang, Yachun An, Chao Ye, Ziyi Liu, Jiangang Gao* , Jianming Yang* , Jiangang Fan* and Renjie Chai* Mechanism and prevention of ototoxicity induced by aminoglycosides.  Front. Cell. Neurosci (第一作者）2021 Jun 15;15:692762. doi: 10.3389/fncel.2021.692762. IF=5.505

 Xiaolong Fu#，Yachun An#, Hongyang Wang#, Peipei Li, Jing Lin, Jia Yuan, Rongyu Yue, Yecheng Jin, Jiangang Gao*, Renjie Chai* Deificiency of Klc2 induces low-Frequency sensorineural hearing loss in C57BL/6J mice and human. Molecular Neurobiology (第一作者）doi: 10.1007/s12035-021-02422-w  IF=5.590（ESI高被引论文，热点论文）

 Peipei Li#, Zhuchun Lin, Yachun An, Jing Lin, Aizhen Zhang, Hailong Tu, Shuangyan Wang, Jie Ran , Jinpeng Wang, Yu Liang, Ziyi Liu, Chao Ye, Xiaolong Fu*, Jiangang Gao* Piccolo is Essential for the Maintenance of Mouse Retina But Not Cochlear Hair Cell Function Aging-US 010522 (并列通讯）IF=5.6817

Jun Lv#, Xiaolong Fu#, Yige Li, Guodong Hong, Peipei Li, Jing Lin, Youfang Xun, Lucheng Fang, Weibin Weng, Rongyu Yue, Geng-Lin Li, Bing Guan*, He Li*, Yideng Huang* and Renjie Chai* Deletion of Kcnj16 in Mice Does Not Alter Auditory Function Front. Cell Dev. Biol doi.org/10.3389/fcell.2021.630361（并列一作）IF=6.684（ESI高被引论文）

Zhenhua Zhong#, Xiaolong Fu#, He Li#, Jie Chen#, Maohua Wang, Song Gao, Liyan Zhang, Cheng Cheng, Shasha Zhang, Xiaoyun Qian, Yilai Shu*, Renjie Chai3* and Xia Gao1, 2* Citicoline protects auditory hair cells against neomycin-induced damage. Front. Cell Dev. Biol. doi:10.3389/fcell.2020.00712（并列一作）IF=6.684

Zongzhuang Wen#, Haixia Zhu#, Zhenzu L#i, Sen Zhang, Aizhen Zhang, Tingting Zhang, Xiaolong Fu , Daqing Sun*,Jian Zhang * Jiangang Gao *A knock-in mouse model of Pendred syndrome with Slc26a4 L236P mutation  Biochemical and Biophysical Research Communications Volume 515, Issue 2, 23 July 2019, Pages 359-365 IF=2.8

Yecheng Jin#, Xiaotong Gao, Xiaolong Fu, Shiwei Li, Xiaoyang Sun, Jiangang Gao*Deletion of Brg1 causes stereocilia bundle fusion and cuticular plate loss in vestibular hair cells. Hearing Research 377 (2019) 247e259  IF=2.9

 Xiaolong Fu#, Renjie Chai Regulation of autophagy: a promising therapeutic target for the treatment of hearing loss.  Journal of Bio-X Research  （第一作者) 2019

 Xiaolong Fu, Xiaoyang Sun, Linqing Zhang, Yecheng Jin, Renjie Chai,  Lili Yang, Xiangguo Liu, Xiaochun Bai, Jianfeng Li, Haibo Wang*, and Jiangang Gao*Tuberous sclerosis complex-mediated m-TORC1 Overactivation Promotes Age-related hearing loss. The Journal of Clinical Investigation. 2018 Nov 1; 128(11):4938-4955（第一作者）IF-14.808(ESI高被引论文）

 Xiaolong Fu, Linqing Zhang, Yecheng Jin, Xiaoyang Sun, Aizhen Zhang, Zongzhuang Wen, Yichen Zhou, Ming Xia* and Jiangang Gao*. Loss of MYH14 Increases Susceptibility to Noise-Induced Hearing loss (NIHL) in CBA/CaJ Mice.  Neural Plasticity. 2016;2016:6720420.（第一作者）IF=3.5（ESI高被引论文）

 Yecheng Jin, Naixia Ren, ShiWei Li, Xiaolong Fu, Xiaoyang Sun, Yuqin Men, Zhigang Xu,Jian Zhang, Yue Xie, Ming Xia* and Jiangang Gao*. Deletion of Brg1 causes abnormal hair cell planer polarity, hair cell anchorage, and scar formation in mouse cochlea. Scientific Reports. 2016 Jun 3;6:27124. doi: 10.1038/srep27124.IF=4.2

Yuqin Men#, Xiujuan Li, Hailong Tu, Aizhen Zhang, Xiaolong Fu, Zhishuo Wang, Yecheng Jin, Congzhe Hou, Tingting Zhang, Sen Zhang, Yichen Zhou, Boqin Li, Jianfeng Li, Xiaoyang Sun*, Haibo Wang*, Jiangang Gao*Tprn is essential for the integrity of stereociliary rootlet in cochlear hair cells in mice. Frontiers of Medicine. 2018 Aug 30. doi: 10.1007/s11684-018-0638-8.IF=1.4

Peipei Li#, Zongzhuang Wen, Guangkai Zhang, Aizhen Zhang, Xiaolong Fu, and Jiangang Gao*Knock-In Mice with Myo3a Y137C Mutation Displayed Progressive Hearing Loss and Hair Cell Degeneration in the Inner Ear. Neural Plasticity. 2018; 2018: 4372913.IF=3.5

Lingcui Ding#, Qianqian Gong, Shiwei Li, Xiaolong Fu, Yecheng Jin, Jian Zhang, Jiangang Gao and Xiaoyang Sun * Rcan2 and estradiol independently regulate body weight in female mice Oncotarget  2017; 8:48098-48109.

专硕培养方向：生物学   校外合作导师：高下 教授 邮箱：xiagao@aliyun.com

高下 简历：

1982-1987 |第四军医大学学士

1992-1995 |第四军医大学硕士

1999-2002 |第四军医大学博士

研究方向：感音神经性耳聋致病机制研究。

成果：

1. Cheng Cheng#, Yilin Hou#, Zhonghong Zhang#，Yanfei Wang#, Ling Lu#,Liyan Zhang, Pei Jiang, Song Gao, Qiaojun Fang, Chengwen Zhu, Junyan Gao, Xufeng Liu, Wei Xie, Zhengping Jia, Zhigang Xu*, Xia Gao*, Renjie Chai*. Disruption of the autism-related gene Pak1 causes stereocilia disorganization, hair cell loss, and deafness in mice. Journal of Genetics and Genomics, 10.1016/j.jgg.2021.03.010, 2021

2. Zhu, G.J.#, Gong, S.#, Ma, D.B.#, Tao, T.#, He, W.Q.#, Zhang, L., Wang, F., Qian, X.Y., Zhou, H., Fan, C., Wang, P., Chen, X., Zhao, W., Sun, J., Chen, H., Wang, Y., Gao, X., Zuo, J., Zhu, M.S.*, Gao, X.*, Wan, G. * (2020). Aldh inhibitor restores auditory function in a mouse model of human deafness. PLOS Genetics, 16(9):e1009040.

3. Han Zhou, Xiaoyun Qian, Nana Xu, Shasha Zhang, Guangjie Zhu, Yuan Zhang, Dingding Liu, Cheng Cheng, Xiaocheng Zhu, Yongze Liu, Ling Lu, Jie Tang, Renjie Chai, Xia Gao. Disruption of Atg7-dependent autophagy causes electromotility disturbances, outer hair cell loss, and deafness in mice. Cell Death Dis, 2020 Oct 24;11(10):913

4. Zhenhua Zhong#, Xiaolong Fu#, He Li#, Jie Chen#, Maohua Wang, Song Gao, Liyan Zhang, Cheng Cheng, Yuan Zhang, Peipei Li, Shasha Zhang, Xiaoyun Qian, Yilai Shu*, Renjie Chai*, Xia Gao*. Citicoline Protects Auditory Hair Cells Against Neomycin-Induced Damage. Frontiers in Cell and Developmental Biology, 8:712, 2020

5. Cheng, C.; Wang, Y. F.; Guo, L.; Lu, X. L.; Zhu, W. J.; Muhammad, W.; Zhang, L. Y.; Lu, L.; Gao, J. Y.; Tang, M. L.; Chen, F. Y.; Gao, X.; Li, H. W.; Chai, R. J., Age-related transcriptome changes in Sox2+supporting cells in the mouse cochlea. Stem Cell Res Ther 2019, 10 (1).

6. Gao, S.; Cheng, C.; Wang, M.; Jiang, P.; Zhang, L.; Wang, Y.; Wu, H.; Zeng, X.; Wang, H.; Gao, X.; Ma, Y.; Chai, R., Blebbistatin Inhibits Neomycin-Induced Apoptosis in Hair Cell-Like HEI-OC-1 Cells and in Cochlear Hair Cells. Frontiers in cellular neuroscience 2019, 13, 590

7. Guo, R. R.; Ma, X. F.; Liao, M. H.; Liu, Y.; Hu, Y. N.; Qian, X. Y.; Tang, Q. L.; Gao, X.; Chai, R. J.; Gao, X.; Tang, M. L., Development and Application of Cochlear Implant-Based Electric-Acoustic Stimulation of Spiral Ganglion Neurons. AcsBiomaterSciEng 2019, 5 (12), 6735-6741.

8. He, Z. H.; Fang, Q. J.; Li, H.; Shao, B. W.; Zhang, Y.; Zhang, Y. H.; Han, X.; Guo, R. R.; Cheng, C.; Guo, L. N.; Shi, L. S.; Li, A.; Yu, C. J.; Kong, W. J.; Zhao, C. J.; Gao, X.; Chai, R. J., The role of FOXG1 in the postnatal development and survival of mouse cochlear hair cells. Neuropharmacology 2019, 144, 43-57.

Zhang, Y. Q.; Li, W.; He, Z. H.; Wang, Y. F.; Shao, B. W.; Cheng, C.; Zhang, S. S.; Tang, M. L.; Qian, X. Y.; Kong, W. J.; Wang, H.; Chai, R. J.; Gao, X., Pre-treatment With Fasudil Prevents Neomycin-Induced Hair Cell Damage by Reducing the Accumulation of Reactive Oxygen Species. Frontiers in molecular neuroscience 2019, 12.

研究课题：

1. 国家自然科学基金面上项目，PINK1/Parkin介导的线粒体自噬在新霉素诱导的毛细胞损伤中的作用及机制，81970884, 2020-01至 2023-12,55万元；

2. 国家自然科学基金面上项目，研究人工耳蜗电刺激对于神经干细胞再生听觉神经元的调控规律及作用机制，81771019, 2018-01至 2021-12,54万元；

3. 国家自然科学基金面上项目，PAK1在噪声损伤模型中对突触再生的作用及其机制，81570921 ，2016/01-2019/12，58万；