1. 国家自然科学基金面上项目，靶向肝癌DNA损伤应答病理性异常扰动的单分子检测技术研究，50W，2025.1-2028.12，负责人：王黎娟。

2. 国家自然科学基金青年基金，DNA糖基化酶的单分子检测及其在癌症早期诊断中的应用研究，25W，2018.1-2020.12，负责人：王黎娟。

3. 国家自然科学基金重点项目，靶向肺癌循环肿瘤细胞的单分子检测技术研究，300W，2018.7-2022.12，参与。

4. 国家自然科学基金青年基金，基于核酸恒温扩增技术的组蛋白修饰超灵敏定量分析方法，25W，2018.1-2020.12，参与。

5. 国家自然科学基金青年基金，稀有核酸碱基的光化学反应动力学机理研究，20W，2017.1-2019.12，参与。

6. 国家自然科学基金青年基金，基于等温扩增的脱氧核酶荧光生物传感器检测肿瘤标志物技术的研究，20W，2017.1-2019.12，参与。

7. 国家自然科学基金面上项目，表面增强拉曼光谱及成像技术在肿瘤细胞检测中的应用研究，65W，2016.1-2019.12，参与。

8. 山东省自然科学基金青年基金，组蛋白修饰酶的超灵敏检测及其在癌症早期诊断中的应用，15W，2016.1-2018.12，参与。

科研奖励：

1. 山东省自然科学奖，二等奖（3/4），山东省人民政府，2022.12.1。

2. 山东省自然科学奖，二等奖（2/4），山东省人民政府，2017.5.5。

3. 山东省大学生科技创新大赛（1/1），二等奖，山东省教育厅，2017.12。

4. 山东省大学生科技创新大赛（1/2），二等奖，山东省教育厅，2018.12。

5. 山东省研究生优秀科技创新成果奖，三等奖，山东省教育厅，2018.11.18。

SCI一作/通讯论文发表：

42. Yao Jiang, Tao Wang, Li-xue Qiao, Li-juan Wang,* and Chun-yang Zhang.* Construction of an end-repairing-engineered quadratic in vitro transcription machine for single-molecule monitoring of alkaline phosphatase in human cancers. Talanta, 2025, 283, 127104. (IF: 5.6) (JCR一区)

41. Shi Wang, Hao Liu, Qinfeng Xu, Li-juan Wang*, and Chun-yang Zhang.* Selective non-Watson-Crick pairing-guided DNA synthesis for locus-specific analysis of 8-oxo-7,8-dihydroguanine in telomeres at ultrahigh resolution. Sensors and Actuators B: Chemical, 2024, 417, 136122. (IF: 8.4) (JCR一区)

40. Li-juan Wang, Wen-jing Liu, Lu-yao Wang, Yun Han, Dong-ling Li, and Chun-yang Zhang.* Construction of an enzymatically controlled DNA nanomachine for one-step imaging of telomerase in living cells. Analytical Chemistry, 2024, 96(11): 4647-4656. (IF: 7.4) (JCR一区)

39. Li-juan Wang, Qian Liu, Ying-ying Lu, LeLiang, and Chun-yang Zhang.* Silver-coordinated Watson-Crick pairing-driven three-dimensional DNA walkers for selective detection of genomic N6‑methyladenine and N4‑methylcytosineat single-molecule level. Analytical Chemistry, 2024, 96(5): 2191-2198. (IF: 7.4) (JCR一区)

38. Li-juan Wang, Xiao-wen Liu, Xinyi Zhang,Yun Han, Qian Liu, Zi-yue Wang,* and Chun-yang Zhang.* Construction of a multiple ligation-driven exponentially symmetric T7-transcription machinery for single-molecule monitoring of diverse single-nucleotide polymorphisms in human cancers. Chemical Engineering Journal, 2024, 158, 148251. (IF: 15.1) (JCR一区)

37. Wen-jing Liu, Li-juan Wang*, and Chun-yang Zhang.* Progress in quantum dot based biosensors for microRNA assay: A review. Analytica Chimica Acta, 2023, 1278, 341615. (IF: 6.2) (JCR一区)

36. Li-juan Wang, Qian Liu, Fei Ma,* and Chun-yang Zhang.* Target-responsive DNAzyme probes for luminescence detection and imaging of DNA-modifying enzymes. TrAC Trends in Analytical Chemistry, 2023, 167, 117270. (IF: 13.1) (JCR一区)

35. Huimin Yuan, Shi Wang, Li-juan Wang*, and Chun-yang Zhang.* A CpG methylation-powered dynamic three-dimensional-DNAzyme walker for single-molecule monitoring of multiple cytosine-C5 methyltransferases. Sensors and Actuators B: Chemical, 2023, 390, 133959. (IF: 9.221) (JCR一区)

34. Meng Liu, Nan Zhong, Xiaorui Tian, Li-juan Wang*, and Chun-yang Zhang.* Programmable strand displacement-driven assembly of single quantum-dot nanosensor for accurate monitoring of human SMUG1 uracil-DNA glycosylase at the single-cell level. Sensors and Actuators B:Chemical, 2023, 382,133568. (IF: 9.221) (JCR一区)

33.  Li-juan Wang, Meng-meng Lv, Jin-ping Hu, Meng Liu, and Chun-yang Zhang.* Proximity ligation-transcription circuits-powered exponential amplifications for single-molecule monitoring of telomerase in human cells. Chemical Communications, 2023, 59(9): 1181-1184. (IF: 6.065) (JCR二区) (Nature Index)

32. Meng Liu, Di Zhang, Jin-ping Hu, Li-juan Wang,* Jian-Ge Qiu* and Chun-yang Zhang.* Construction of bidirectional strand displacement-driven three-dimensional DNA walkers for single-molecule monitoring of multiple DNA glycosylases. Sensors and Actuators B: Chemical, 2023, 380, 133357. (IF: 9.221) (JCR一区)

31. Li-juan Wang, Li-ping Pan, Xiaoran Zou, Jian-Ge Qiu,* and Chun-yang Zhang.* Activatable self-dissociating of Watson-Crick structures with fluorescent nucleotides for multiple human glycosylases sensing at the single-cell level. Analytical Chemistry, 2022, 94(50): 17700-17708. (IF: 8.008) (JCR一区) (Nature Index)

30. Li-juan Wang, Qian Han, Jin-ping Hu, Hou-xiu Wang, and Chun-yang Zhang.* Structure-switchable hairpins-powered exponential replications for sensing attomolar microRNA single nucleotide polymorphisms in human cancer tissues with zero background. Analytical Chemistry, 2022, 94(44): 15171-15175. (IF: 8.008) (JCR一区) (Nature Index)

29. Li-juan Wang, Hao Liu, Xiao-Fang Li, Yingying Meng, Jian-Ge Qiu,* and Chun-yang Zhang.* Methylation-powered engineering of a dual-color light-up RNA nanosensor for label-free and ultrasensitive sensing of multiple DNA methyltransferases. Sensors and Actuators B: Chemical, 2022, 371, 132524. (IF: 9.221) (JCR一区)

28. Ming-hao Liu, Wan-tong Yu,Meng Liu, Yan Zhang, Li-juan Wang,* and Chun-yang Zhang.* Enzymatic DNA repair cascade-driven fluorophore encoding for sensitively sensing telomerase activity in cancer cells. Sensors and Actuators B: Chemical, 2022, 359(15): 131603. (IF: 9.221) (JCR一区)

27. Li-juan Wang, Qian Han, Jian-Ge Qiu, and Chun-yang Zhang.* Cooperative in-situ assembly of G-quadruplex DNAzyme nanowires for one-step sensing of CpG methylation in human genomes. Nano Letters, 2022, 22(1): 347-354. (IF: 12.262) (JCR一区)

26. Li-juan Wang, Hao Liu, Xiaoran Zou, Qinfeng Xu, and Chun-yang Zhang.* 3'-Terminal repair-powered dendritic nanoassembly of poly-adenine molecular beacons for one-step quantification of alkaline phosphatase in human serum. Analytical Chemistry, 2021, 93(30):10704-10711.  (IF: 8.008)(JCR一区) (Nature Index)

25. Li-juan Wang, Le Liang, Jian-Ge Qiu, BingHua Jiang,* and Chun-yang Zhang.* A controlled T7 transcription-driven symmetric amplification cascade machinery for single-molecule detection of multiple repair glycosylases. Chemical Science, 2021, 12(15):5544-5554. (IF: 9.969) (JCR一区) (Nature Index)

24. Yueying Li, Shuli Sun, Xiaorui Tian, Jian-Ge Qiu, BingHua Jiang, Li-juan Wang,* and Chun-yang Zhang.* Dephosphorylation-directed tricyclic DNA amplification cascades for specific and sensitive detection of protein tyrosine phosphatase. Chemical Communications, 2020, 56(78): 11581-11584. (IF: 6.065) (JCR二区) (Nature Index) (Cover article)

23. Li-juan Wang, Xiao Han, Jian-Ge Qiu, BingHua Jiang,* and Chun-yang Zhang.* Cytosine-5 methylation-directed construction of Au nanoparticle-based nanosensor for simultaneous detection of multiple DNA methyltransferases at the single-molecule level. Chemical Science, 2020, 11(35): 9675-9684. (IF: 9.969) (JCR一区) (Nature Index)

22. Li-juan Wang, Ming Ren, Hou-xiu Wang,Jian-Ge Qiu, BingHua Jiang,* and Chun-yang Zhang.* Construction of a quencher-free cascade amplification system for highly specific and sensitive detection of serum circulating miRNAs. Analytical Chemistry, 2020, 92(12): 8546-8552. (IF: 8.008) (JCR一区) (Nature Index)

21. Li-juan Wang, Ying-ying Lu, and Chun-yang Zhang.* Construction of self-directed replication system for label-free and real-time sensing of repair glycosylases with zero background. Chemical Science, 2020, 11(2), 587-595. (IF: 9.969) (JCR一区) (Nature Index)

20. Chen-chen Li, Zi-yue Wang, Li-juan Wang*, and Chun-yang Zhang.* Biosensors for epigenetic biomarkers detection: a review. Biosensors and Bioelectronics, 2019, 144, 111695-111705. (IF: 12.545) (JCR一区)

19. Li-juan Wang, Ming-li Luo, Xiao-yun Yang, Xiao-fang Li, Yanxia Wu, and Chun-yang Zhang.* Controllable autocatalytic cleavage-mediated fluorescence recovery for homogeneous sensing of alkyladenine DNA glycosylase from human cancer cells. Theranostics, 2019, 9(15): 4450-4460. (IF: 11.6) (JCR一区)

18. Huige Zhang, Li-juan Wang, Lili Wang, Hongli Chen,* Xingguo Chen, and Chun-yang Zhang.* Development of cascade isothermal amplification approach for sensitive detection of DNA methyltransferase. Journal of Materials Chemistry B, 2019, 7(1): 157-162. (IF: 7.571) (JCR一区) (co-first author)

17. Li-juan Wang, Hou-xiu Wang, Longhe Jiang, and Chun-yang Zhang.* Development of an in vitro autocatalytic self-replication system for biosensing application. ACS Sensors, 2018, 3 (12): 2675-2683. (IF: 9.618) (JCR一区)

16. Li-juan Wang, Zi-yue Wang, and Chun-yang Zhang.* Primer dephosphorylation-initiated circular exponential amplification for ultrasensitive detection of alkaline phosphatase. Analyst, 2018, 143(19): 4606-4613. (IF: 5.227) (JCR一区) (Hot paper)

15. Li-juan Wang, Xiao Han, Chen-chen Li, and Chun-yang Zhang.* Single-ribonucleotide repair-mediated ligation-dependent cycling signal amplification for sensitive and specific detection of DNA methyltransferase. Chemical Science, 2018, 9(28): 6053-6061. (IF: 9.969) (JCR一区)

(Nature Index) (Cover article)

14. Li-juan Wang, Ming Ren, Li Liang, and Chun-yang Zhang.* Controllable fabrication of bio-bar codes for dendritically amplified sensing of human T-lymphotropic virus. Chemical Science, 2018, 9(22): 4942-4949. (IF: 9.969) (JCR一区) (Nature Index) (Cover article)

13. Zi-yue Wang, Li-juan Wang, Qianyi Zhang, Bo Tang,* and Chun-yang Zhang.* Single quantum dot-based nanosensor for sensitive detection of 5-methylcytosine at both CpG and non-CpG sites. Chemical Science, 2018, 9(5): 1330-1338. (IF: 9.969) (JCR一区) (Nature Index) (co-first author)

12. Li-juan Wang, Ming-li Luo, Qianyi Zhang, Bo Tang,* and Chun-yang Zhang.* Single quantum dot-based nanosensor for rapid and sensitive detection of terminal deoxynucleotidyl transferase. Chemical Communications, 2017, 53(80): 11016-11019. (IF:6.065) (JCR二区) (Nature Index)

11. Li-juan Wang, Qianyi Zhang, Bo Tang,* and Chun-yang Zhang.* Single-molecule detection of polynucleotides kinase based on phosphorylation-directed recovery of fluorescence quenched by Au nanoparticles. Analytical Chemistry, 2017, 89(13): 7255-7261. (IF: 8.008) (JCR一区) (Nature Index)

10. Li-juan Wang, Ming Ren, Qianyi Zhang, Bo Tang,* and Chun-yang Zhang.* Excision repair-initiated enzyme-assisted bicyclic cascade signal amplification for ultrasensitive detection of uracil-DNA glycosylase. Analytical Chemistry, 2017, 89(8): 4488-4494. (IF: 8.008) (JCR一区) (Nature Index)

9. Li-juan Wang, Fei Ma, Bo Tang,* and Chun-yang Zhang.* Sensing telomerase: from in vitro detection to in vivo imaging. Chemical Science, 2017, 8(4): 2495-2502. (IF: 9.969) (JCR一区) (Nature Index)

8. Li-juan Wang, Zi-yue Wang, Qianyi Zhang, Bo Tang,* and Chun-yang Zhang.* Cyclic enzymatic repairing-mediated dual-signal amplification for real-time monitoring of thymine DNA glycosylase. Chemical Communications, 2017, 27(53): 3878-3881. (IF: 6.065) (JCR二区) (Nature Index)

7. Li-juan Wang, Fei Ma, Bo Tang,* Chun-yang Zhang.* Base-excision-repair-induced construction of a single quantum-dot-based sensor for sensitive detection of DNA glycosylase activity. Analytical Chemistry, 2016, 88(15): 7523-7529. (IF: 8.008) (JCR一区) (Nature Index)

6. Li-juan Wang, Yong Yang, Chun-yang Zhang*. Phosphorylation-directed assembly of a single quantum dot based nanosensor for protein kinase assay. Analytical Chemistry, 2015, 87(9): 4696-4703. (IF: 8.008) (JCR一区) (Nature Index)

5. Li-juan Wang, Yan Zhang, Chun-yang Zhang*. A target-triggered exponential amplification-based DNAzyme biosensor for ultrasensitive detection of folate receptors. Chemical Communications, 2014, 50(97): 15393-15396. (IF: 6.065) (JCR二区) (Nature Index)

4. Yan Zhang, Li-juan Wang, Chun-yang Zhang*. Highly sensitive detection of telomerase using a telomere-triggered isothermal exponential amplification-based DNAzyme biosensor. Chemical Communications, 2014, 50(15): 1909-1911. (IF: 6.065) (JCR二区) (Nature Index) (co-first author)

3. Lijuan Wang, Fei Ma, Sheng Xu, Tianqing Zheng, Ren Wang, Huiping Chen, Wenbiao Shen*. Cloning and characterization of a heme oxygenase-2 gene from rice (Oryza sativa L.), and its expression analysis in response to some abiotic stresses. Acta Physiologiae Plantarum, 2014, 36(4): 893-902. (IF: 2.736) (JCR二区)

2. Fei Ma, Lijuan Wang, Jiale Li, Muhammad Kaleem Samma, Yanjie Xie, Ren Wang, Jin Wang, Jing Zhang, Wenbiao Shen*. Interaction between HY1 and H2O2 in auxin-induced lateral root formation in Arabidopsis, Plant Molecular Biology, 2014, 85(1-2): 49-61. (IF: 4.335) (JCR一区)  (co-first author)

1. Li-juan Wang, Yan Zhang, Chun-yang Zhang*. Ultrasensitive detection of telomerase activity at the single-cell level. Analytical Chemistry, 2013, 85(23): 11509-11517. (IF: 8.008) (JCR一区) (Nature Index)

国家授权发明专利：

1. 一种基于协同原位组装G-四链体DNAzyme纳米线的化学发光传感器及其应用，授权号：ZL202111470190.X，授权日期：2024.8.16。

2. 可控自催化切割介导荧光恢复检测hAAG的传感器及方法，授权号：ZL201910343592.X，授权日期：2023.6.2。

3. 一种酪氨酸磷酸酶生物传感器及其检测方法与应用，授权号：ZL202010812925.1，授权日期：2023.5.9。

4. 一种检测DNA甲基化转移酶的纳米生物传感器、其检测方法及应用，授权号：ZL202010831729.9，授权日期：2023.05.05。

5. 一种同时检测hOGG1和hAAG的荧光传感器及其检测方法和应用，授权号：ZL202110076541.2，授权日期：2022.12.27。

6. 基于切除修复由酶介导的两步串联信号放大检测UDG活性的方法，授权号：ZL201710104367.1，授权日期：2018.10.12。

7. 一种基于单个量子点水平检测DNA糖基化酶活性的方法，授权号：ZL201610150395.2，授权日期：2018.10.26。

8. 一种基于循环酶修复介导的双信号放大策略检测胸腺嘧啶DNA糖基化酶活性的方法，授权号：ZL201710166336.9，授权日期：2020.5.5。

9. 基于酶催化可控自组装生物条码检测HTLV-II DNA的方法，授权号：ZL201810444445.7，授权日期：2022.6.10。